Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor.

Abstract

In this study, the cultivation conditions of Prorocentrum lima, including temperature, nutrient concentration, photoperiod, and salinity were observed, and then an effective method for the large-volume cultivation of P. lima using a vertical flat photobioreactor was developed for the first time. The maximum cell concentrations and toxin contents of P. lima cultured in the photobioreactor were reached after a 35 days cultivation. Moreover, a step-wise double-sedimentation centrifugation method was used to harvest the microalgae cells, with the harvest rate of 89%. Toxin analysis of dry microalgal powder indicated that OA and DTX1 contents were 15.2 and 21.6 mg g(-1), respectively. These results verify that the culture method using the proposed photobioreactor is effective to massively produce DSP toxin-containing P. lima. This study may serve as a guide for the large-scale production of toxin-producing red-tide benthic microalgae.

• Authors: Shuai Wang, Junhui Chen, Zhaoyong Li, Yanlong Wang, Boqiang Fu, Xiaotian Han, Li Zheng
• Journal: Bioresource technology (Bioresour Technol) Vol. 179 Pg. 243-248 (Mar 2015) ISSN: 1873-2976 [Electronic] England
• PMID: 25545093 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Marine Toxins
• Powders
• Okadaic Acid

Topics

• Cell Culture Techniques (instrumentation)
• Dinoflagellida (growth & development, metabolism)
• Ecosystem
• Marine Toxins (biosynthesis)
• Mass Spectrometry
• Microalgae (growth & development, metabolism)
• Okadaic Acid (analysis)
• Photobioreactors
• Photoperiod
• Powders
• Shellfish Poisoning (metabolism)
• Temperature