Abstract |
Basal serum 17OHP measurement remains the first screening step for nonclassic congenital adrenal hyperplasia ( NCCAH) and the accuracy of the test is of high value. The aim of this study was to compare the accuracy of immunoassays to LC-MS/MS in the assessment of serum 17OHP and androgens concentration in women with hyperandrogenism and controls. 17OHP, total testosterone, androstendione and DHEA-S were measured in 39 women with clinically and/or biochemically evident hyperandrogenism and in 29 age-matched controls without clinical hyperandrogenism. 17OHP and androgens were measured by immunoassays and by LC-MS/MS. In patients group median 17OHP level measured by immunoassays was significantly higher compared to LC-MS/MS (5.49 nmol/l-ELISA NovaTec® and 3.57 nmol/l-ELISA DRG® versus 1.56 nmol/l-LC-MS/MS p < 0.0001) as well as in the control group (2.58 nmol/l-ELISA DRG® versus 1.14 nmol/l-LC-MS/MS p < 0.0001). Additional, unnecessary diagnostic procedures explaining elevated 17OHP level were undertaken in 85% of patients when NovaTec® test was used, in 50% when ELISA DRG® and in none when LC-MS/MS method was applied. Total testosterone, androstendione and DHEA-S concentrations in the patients and the controls assessed by the immunoassays were also significantly higher compared to LC-MS/MS. LC-MS/MS is more reliable diagnostic tool in the measurement of serum 17OHP and androgens concentrations compared to immunoassays in women with hyperandrogenism.
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Authors | Urszula Ambroziak, Anna Kępczyńska-Nyk, Alina Kuryłowicz, Aleksandra Wysłouch-Cieszyńska, Ewa Maria Małunowicz, Zbigniew Bartoszewicz, Agnieszka Kondracka, Radosław Jaźwiec, Emilia Pawłowska, Magdalena Szcześniak, Michał Dadlez, Tomasz Bednarczuk |
Journal | Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology
(Gynecol Endocrinol)
Vol. 31
Issue 4
Pg. 296-300
(Apr 2015)
ISSN: 1473-0766 [Electronic] England |
PMID | 25539143
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Reagent Kits, Diagnostic
- Testosterone
- Androstenedione
- Dehydroepiandrosterone Sulfate
- 17-alpha-Hydroxyprogesterone
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Topics |
- 17-alpha-Hydroxyprogesterone
(blood)
- Adolescent
- Adrenal Hyperplasia, Congenital
(blood, diagnosis)
- Adult
- Androstenedione
(blood)
- Chromatography, High Pressure Liquid
- Dehydroepiandrosterone Sulfate
(blood)
- Diagnosis, Differential
- False Positive Reactions
- Female
- Hospitals, University
- Humans
- Immunoassay
- Poland
- Reagent Kits, Diagnostic
- Reproducibility of Results
- Sensitivity and Specificity
- Tandem Mass Spectrometry
- Testosterone
(blood)
- Young Adult
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