Abstract | INTRODUCTION: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal-epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124-131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. MATERIALS AND METHODS: RESULTS: We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 ( GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells. CONCLUSIONS: Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.
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Authors | L N Guerra, C Suarez, D Soto, A Schiappacasse, D Sapochnik, P Sacca, G Piwien-Pilipuk, B Peral, J C Calvo |
Journal | Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
(Clin Transl Oncol)
Vol. 17
Issue 7
Pg. 511-20
(Jul 2015)
ISSN: 1699-3055 [Electronic] Italy |
PMID | 25532485
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCAAT-Enhancer-Binding Protein-beta
- Fabp4 protein, mouse
- Fatty Acid-Binding Proteins
- Triglycerides
- Sulfurtransferases
- GAL3ST2 protein, human
- Gal3St2 protein, mouse
- Sulfotransferases
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Topics |
- 3T3-L1 Cells
- Adipocytes
(cytology, metabolism)
- Adipogenesis
- Animals
- CCAAT-Enhancer-Binding Protein-beta
(metabolism)
- Cell Differentiation
- Cell Line, Tumor
- Fatty Acid-Binding Proteins
(metabolism)
- Female
- HEK293 Cells
- Humans
- MCF-7 Cells
- Mammary Glands, Animal
(cytology, metabolism)
- Mice
- NIH 3T3 Cells
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Sulfotransferases
(metabolism)
- Sulfurtransferases
(metabolism)
- Triglycerides
(metabolism)
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