Abstract |
This study evaluated the gastrointestinal microbial diversity and the expression of pattern recognition receptors ( PRRs) of the small intestine during the first week post-weaning in newly weaned piglets. Sixteen piglets were sacrificed on days 0, 1, 4, and 7 post-weaning. Luminal contents from the stomach, ileum, and colon were collected to determine the microbiota diversity; intestinal mucosa from the ileum was collected to assess mRNA expression of PRRs, including toll-like receptors (TLRs) and NOD-like receptors (NLRs); sections of ileum were examined immunohistochemically to assess the immunoglobulin-secreting cells. The results showed that the number of denaturing gradient gel electrophoresis (DGGE) bands from the ileum and colon contents were significantly reduced in the d 4 post-weaning group. Biodiversity indexes (Shannon-Wiener index, richness index, and evenness index) were significantly decreased in the ileum of weaning groups. These indexes decreased in the colon of the d 4 post-weaning group. No significant differences were obtained in the stomach. With the exception of TLR5, the mRNA expressions of TLR2, TLR4, and TLR7 increased post-weaning. The mRNA expressions of NOD1 and NOD2 were significantly affected in the d 4 post-weaning group, and there were no significant differences in the d 1 or d 7 post-weaning groups. Analysis of the immunoglobulin-secreting ( IgA, IgG, and IgM) cells showed that the ratio of each immunoglobulin was significantly higher on d 7 than d 0. The results revealed that microbial diversity was lower in the ileum and on d 4 post-weaning. Weaning significantly affected the expression of intestinal PRRs mainly on d 1 and d 4 post-weaning. The expression of specific PRRs was triggered by weaning to recognize distinctive microbiota and promote the development and maturation of the intestinal mucosal immunity.
|
Authors | Xin Tao, Ziwei Xu, Jing Wan |
Journal | Anaerobe
(Anaerobe)
Vol. 32
Pg. 51-56
(Apr 2015)
ISSN: 1095-8274 [Electronic] England |
PMID | 25528290
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2014 Elsevier Ltd. All rights reserved. |
Chemical References |
- RNA, Ribosomal, 16S
- Receptors, Pattern Recognition
|
Topics |
- Animals
- Antibody Formation
- Biodiversity
- Gastrointestinal Microbiome
(genetics)
- Gene Expression
- Immunity, Mucosal
(genetics, immunology)
- Lymphocyte Count
- Microbiota
- Plasma Cells
(immunology)
- RNA, Ribosomal, 16S
(genetics)
- Receptors, Pattern Recognition
(genetics)
- Swine
- Weaning
|