Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.

Abstract

Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.

• Authors: Angèle Gayet-Ageron, Frédéric Laurent, Jacques Schrenzel, Béatrice Charton, Gisela Jimenez-Getaz, Manuela Tangomo, Tristan Ferry, Patrice Sednaoui, Stephan Lautenschlager, Laurence Toutous-Trellu, Begoña Martinez de Tejada, Matthias Cavassini, Stéphane Emonet, Thomas Perneger, Hélène Salord
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 53 Issue 3 Pg. 976-80 (Mar 2015) ISSN: 1098-660X [Electronic] United States
• PMID: 25520453 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015, American Society for Microbiology. All Rights Reserved.

Chemical References

• 47-kDa immunogen, Treponema pallidum
• Carrier Proteins
• Lipoproteins
• DNA Polymerase I

Topics

• Adult
• Carrier Proteins (analysis, genetics)
• DNA Polymerase I (analysis, genetics)
• Female
• Humans
• Lipoproteins (analysis, genetics)
• Male
• Middle Aged
• Molecular Diagnostic Techniques (methods)
• Polymerase Chain Reaction (methods)
• Prospective Studies
• Syphilis (diagnosis, microbiology)
• Treponema pallidum (genetics, isolation & purification)
• Ulcer (microbiology)