Drug resistance effects of ribosomal protein L24 overexpression in hepatocellular carcinoma HepG2 cells.

Abstract

BACKGROUND:

The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer.

MATERIALS AND METHODS:

Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null- transfected HepG2 cell lines.

RESULTS:

The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation.

CONCLUSIONS:

The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.

• Authors: Yong-Li Guo, Qing-Sheng Kong, Hong-Sheng Liu, Wen-Bin Tan
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 15 Issue 22 Pg. 9853-7 ( 2014) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25520117 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Guanidines
• RNA, Messenger
• Ribosomal Proteins
• ribosomal protein L24
• amycin A

Topics

• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (drug therapy, genetics, pathology)
• Cell Proliferation (drug effects)
• Drug Resistance, Neoplasm (genetics)
• Guanidines (pharmacology)
• Hep G2 Cells
• Humans
• Liver Neoplasms (drug therapy, genetics, pathology)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Ribosomal Proteins (genetics, metabolism)