Patchouli alcohol protects against lipopolysaccharide-induced acute lung injury in mice.

Abstract

BACKGROUND:

Patchouli alcohol (PA), a natural compound isolated from Pogostemon cablin, has been reported to possess anti-inflammatory activity. However, the effects of PA on lipopolysaccharide (LPS)-induced acute lung injury (ALI) have not yet been studied. In the present study, we investigated in vivo the effect of PA on ALI induced by LPS.

METHODS:

Mice were administrated intranasally with LPS to induce lung injury. PA was administrated intraperitoneally 1 h before or after the LPS challenge.

RESULTS:

The results showed that PA significantly decreased the wet-to-dry weight ratio of lungs and the number of total cells, neutrophils, and macrophages in bronchoalveolar lavage fluid at 7 h after the LPS challenge. In addition, PA also suppressed the production of inflammatory cytokines, such as tumor necrosis factor-α, interleukin-1β, and interleukin-6 in bronchoalveolar lavage fluid. Furthermore, Western blot analysis showed that PA inhibited the phosphorylation of IκB-α and p65 nuclear factor κB (NF-κB) induced by LPS.

CONCLUSIONS:

Our results suggest that the anti-inflammatory effects of PA against LPS-induced ALI may be due to its ability to inhibit NF-κB signaling pathways.

• Authors: Jin-Long Yu, Xiao-Shi Zhang, Xia Xue, Rong-Mei Wang
• Journal: The Journal of surgical research (J Surg Res) Vol. 194 Issue 2 Pg. 537-543 (Apr 2015) ISSN: 1095-8673 [Electronic] United States
• PMID: 25519986 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Cytokines
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Sesquiterpenes
• Peroxidase
• patchouli alcohol

Topics

• Acute Lung Injury (drug therapy, metabolism, pathology)
• Animals
• Bronchoalveolar Lavage Fluid (chemistry, immunology)
• Cytokines (analysis, metabolism)
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Lamiaceae
• Lipopolysaccharides
• Lung (drug effects, metabolism, pathology)
• Male
• Mice, Inbred BALB C
• NF-kappa B (metabolism)
• Peroxidase (metabolism)
• Phytotherapy
• Plant Extracts (pharmacology, therapeutic use)
• Random Allocation
• Sesquiterpenes (pharmacology, therapeutic use)