Bioactive DOPA melanin isolated and characterised from a marine actinobacterium Streptomyces sp. MVCS6 from Versova coast.

Abstract

The melanin pigment produced from Streptomyces sp., MVCS6 was isolated and dihydroxyphenyalanine (DOPA) melanin compound was biochemically identified and spectroscopically characterised (ultraviolet and FT-IR). DOPA melanin showed a promising activity as an antibacterial natural product against 12 pathogenic bacteria from hospital isolations, particularly, against Pseudomonas aeruginosa RMMH7 (inhibition zone of 18 ± 0.02 at 30 μg/disc, and MIC of 10 ± 0.02 μg/mL) and Vibrio parahaemolytics RMMH12 (inhibition zone of 15 mm ± 0.03 at 30 μg/disc, and MIC of 14 ± 0.02 μg/mL). Moreover, in vitro evaluation of reducing power (Ascorbic Acid Equivalent (160 μg/mL)), DPPH radical-scavenging (89%), NO-scavenging (72%) and lipid peroxidation activities (89.6%) were determined. Cytotoxicity of DOPA melanin against cervical cancer cell line showed a dose-response activity, and IC50 value was found to be 300 μg/mL. These results would open the way to propose Streptomyces sp. MVCS6 as a promising source of bioactive eumelanin with therapeutic potential in medicine.

• Authors: P Sivaperumal, K Kamala, R Rajaram
• Journal: Natural product research (Nat Prod Res) Vol. 29 Issue 22 Pg. 2117-21 ( 2015) ISSN: 1478-6427 [Electronic] England
• PMID: 25518869 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Antioxidants
• Dihydroxyphenylalanine
• DOPA melanin

Topics

• Anti-Bacterial Agents (chemistry, isolation & purification)
• Antioxidants (chemistry, isolation & purification)
• Cell Line, Tumor (drug effects)
• Dihydroxyphenylalanine (analogs & derivatives, chemistry, isolation & purification)
• Humans
• India
• Microbial Sensitivity Tests
• Pseudomonas aeruginosa (drug effects)
• Seawater (microbiology)
• Streptomyces (chemistry)
• Vibrio parahaemolyticus (drug effects)