Carnosic acid attenuates apoptosis induced by amyloid-β 1-42 or 1-43 in SH-SY5Y human neuroblastoma cells.

Abstract

Amyloid-beta (Aβ) peptides, Aβ 1-42 (Aβ42) and Aβ43 in particular, cause neurotoxicity and cell death in the brain of Alzheimer's disease (AD) at higher concentrations. Carnosic acid (CA), a phenolic diterpene compound in the labiate herbs rosemary and sage, serves as an activator for neuroprotective and neurotrophic functions in brain cells. We investigated the effect of CA on apoptosis induced by Aβ42 or Aβ43 in cultured SH-SY5Y human neuroblastoma cells. Treatment of the cells with Aβ42 or Aβ43 (monomer, 10 μM each) induced apoptosis, which was confirmed by the cleavage of poly-(ADP-ribose) polymerase (PARP) and apoptosis-inducing factor (AIF). Concurrently, the Aβ treatment induced the activation of caspase (Casp) cascades including an effector Casp (Casp3) and initiator Casps (Casp4, Casp8 and Casp9). Pretreatment of the cells with CA (10 μM) partially attenuated the apoptosis induced by Aβ42 or Aβ43. CA pretreatment also reduced the cellular oligomers of Aβ42 and Aβ43. These results suggest that CA suppressed the activation of Casp cascades by reducing the intracellular oligomerization of exogenous Aβ42/43 monomer. The ingestion of an adequate amount of CA may have a potential in the prevention of Aβ-mediated diseases, particularly AD.

• Authors: Pengfei Meng, Hidemi Yoshida, Kunikazu Tanji, Tomoh Matsumiya, Fei Xing, Ryo Hayakari, Liang Wang, Kazushi Tsuruga, Hiroshi Tanaka, Junsei Mimura, Kunio Kosaka, Ken Itoh, Ippei Takahashi, Shogo Kawaguchi, Tadaatsu Imaizumi
• Journal: Neuroscience research (Neurosci Res) Vol. 94 Pg. 1-9 (May 2015) ISSN: 1872-8111 [Electronic] Ireland
• PMID: 25510380 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

Chemical References

• Abietanes
• Amyloid beta-Peptides
• Antioxidants
• Peptide Fragments
• Plant Extracts
• amyloid beta-protein (1-42)
• amyloid beta-protein (1-43)
• Poly(ADP-ribose) Polymerases
• Caspases
• salvin

Topics

• Abietanes (pharmacology)
• Amyloid beta-Peptides (pharmacology)
• Antioxidants (pharmacology)
• Apoptosis (drug effects)
• Caspases (metabolism)
• Cell Line, Tumor
• Cell Survival
• Dose-Response Relationship, Drug
• Humans
• Neuroblastoma (pathology)
• Peptide Fragments (pharmacology)
• Plant Extracts (pharmacology)
• Poly(ADP-ribose) Polymerases (metabolism)
• Time Factors