RORα inhibits adipocyte-conditioned medium-induced colorectal cancer cell proliferation and migration and chick embryo chorioallantoic membrane angiopoiesis.

Abstract

Lipid metabolic disturbances are related to many diseases, such as obesity, diabetes, and certain cancers. Notably, lipid metabolic disturbances have been reported to be a risk factor for colorectal cancer. Nuclear receptors act as ligand-dependent transcription regulators and play key roles in the regulation of body lipid metabolism and the development of many cancers. Retinoic acid receptor-related orphan receptor α (RORα) is a nuclear receptor and can regulate several lipid metabolism genes in certain cancers. Herein, we demonstrate that the conditioned medium from adipocytes has a proproliferative and promigratory effect on colorectal cancer cells and enhances angiogenesis in chicken embryonic chorioallantoic membranes. In addition, the conditioned medium leads to a decrease in the expression of RORα and its target genes. Meanwhile, RORα and its target gene expressions are lower in human colorectal tumor tissue compared with control colorectal tissue. Activation of RORα inhibits the effect of conditioned medium on the proliferation and migration of colorectal cancer cells as well as the angiogenesis in chicken embryonic allantoic membranes. In colorectal cancer cells, the putative ligand of RORα, cholesterol sulfate (CS), prevents cell cycle progression at the G1/S boundary and concurrently modulates the expression of cell cycle-regulatory genes in colorectal cancer cell. CS inhibits angiogenesis in chicken embryonic chorioallantoic membranes and concurrently decreases the mRNA expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α as well as the secretion of VEGF. In addition, lipogenic gene expression is higher in human colorectal tumor tissue compared with control colorectal tissue. CS inhibits the expression of lipogenic genes in colorectal cancer cells. These results suggest that RORα could represent a direct link between local lipid metabolism of colorectal tissue and colorectal cancer. Therefore, the reduction of the expression of RORα could represent a potential warning sign of colorectal cancer.

• Authors: Lei Xiao, Jing Wang, Jiao Li, Xiongwei Chen, Pengfei Xu, Suozhu Sun, Dacheng He, Yusheng Cong, Yonggong Zhai
• Journal: American journal of physiology. Cell physiology (Am J Physiol Cell Physiol) Vol. 308 Issue 5 Pg. C385-96 (Mar 01 2015) ISSN: 1522-1563 [Electronic] United States
• PMID: 25500738 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 the American Physiological Society.

Chemical References

• Culture Media, Conditioned
• Nuclear Receptor Subfamily 1, Group F, Member 1
• RORA protein, human

Topics

• 3T3 Cells
• Adipocytes (physiology)
• Animals
• Cell Line, Tumor
• Cell Movement (drug effects, physiology)
• Cell Proliferation (drug effects, physiology)
• Cell Survival (drug effects, physiology)
• Chick Embryo
• Chorioallantoic Membrane (blood supply, drug effects, physiology)
• Colorectal Neoplasms (metabolism, pathology)
• Culture Media, Conditioned (pharmacology)
• Humans
• Mice
• Neovascularization, Physiologic (drug effects, physiology)
• Nuclear Receptor Subfamily 1, Group F, Member 1 (antagonists & inhibitors, biosynthesis)