Analysis of breast cancer-associated glycosphingolipids using electrospray ionization-linear ion trap quadrupole mass spectrometry.

Abstract

Abnormal glycosphingolipids (GSLs) are expressed in many human tumors. These tumor-associated GSLs may have important roles in tumor progression. However, they are hard to be detected because of their low concentration and the limited availability of antibodies and lectins that recognize them. Thus, mass spectrometry is an effective method to analyze GSLs with high sensitivity. Here, we use electrospray ionization-linear ion trap quadrupole mass spectrometry (ESI-LTQ-MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) to determine the structure of a certain GSL in human breast cancer tissue. To obtain the breast cancer-associated GSLs, we applied relative abundance contrast of GSLs and signal-to-noise ratio (SNR) analyses. We also used α1,2 fucosidase and fucosyltransferases as tools in the structural analysis. Based on this analysis, we identified the ion with m/z 1184 molecular ion as fucosyl-lactoceramide (Fuc-LacCer) with a C16 fatty acid ceramide. Quantitative analysis of GSLs revealed both Fuc-LacCer and Globo-H increased in breast cancer tissues. However, these two breast cancer-associated GSLs had different roles. The results of SNR analysis suggested the abnormal Fuc-LacCer is specific to breast cancer. The GSL profiling of breast cancer cells showed fucosyltransferase 1 contributed to the biosynthesis of Globo-H and Fuc-LacCer. In conclusion, MS analysis identified an accumulation of Fuc-LacCer in breast cancer tissue. Our findings provide GSL profiles of human breast cancer and develop an MS method for the study of cancer-associated GSLs.

• Authors: Tingting Zhu, Longjiang Xu, Xiukun Xu, Zheng Wang, Jian Zhu, Qing Xie, Biyan Zhang, Yanping Wang, Linling Ju, Yun He, Xinshan Ye, Dapeng Zhou, Yunsen Li
• Journal: Carbohydrate research (Carbohydr Res) Vol. 402 Pg. 189-99 (Jan 30 2015) ISSN: 1873-426X [Electronic] Netherlands
• PMID: 25498019 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd. All rights reserved.

Chemical References

• Glycosphingolipids
• Fucosyltransferases

Topics

• Adult
• Aged
• Aged, 80 and over
• Breast Neoplasms (enzymology, metabolism, pathology)
• Cell Line, Tumor
• Female
• Fucosyltransferases (metabolism)
• Gene Expression Regulation, Neoplastic
• Glycosphingolipids (metabolism)
• Humans
• Middle Aged
• Spectrometry, Mass, Electrospray Ionization