Phosphorescent ruthenium complexes with a nitroimidazole unit that image oxygen fluctuation in tumor tissue.

Abstract

Understanding oxygen fluctuation in a cancerous tumor is important for effective treatment, especially during radiotherapy. In this paper, ruthenium complexes bearing a nitroimidazole group are shown to report the oxygen status in tumor tissue directly. The nitroimidazole group was known to be accumulated in hypoxic tumor tissues. On the other hand, the ruthenium complex showed strong phosphorescence around 600 nm. The emission of ruthenium is quenched instantaneously by molecular oxygen due to energy transfer between triplet states of oxygen and ruthenium complex, but the emission is then recovered by the removal of oxygen. Thus, we could observe oxygen fluctuation in tumor tissue in a real-time manner by monitoring the phosphorescence of the ruthenium complex. The versatility of the probe is demonstrated by monitoring oxygen fluctuation in living cells and tumor tissue planted in mice. The ruthenium complex promptly penetrated plasma membrane and accumulated in cells to emit its oxygen-dependent phosphorescence. In vivo experiments revealed that the oxygen level in tumor tissue seems to fluctuate at the sub-minute timescale.

• Authors: Aoi Son, Atsushi Kawasaki, Daiki Hara, Takeo Ito, Kazuhito Tanabe
• Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (Chemistry) Vol. 21 Issue 6 Pg. 2527-36 (Feb 02 2015) ISSN: 1521-3765 [Electronic] Germany
• PMID: 25492852 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Coordination Complexes
• Nitroimidazoles
• Green Fluorescent Proteins
• Ruthenium
• Oxygen

Topics

• Animals
• Cell Hypoxia
• Cell Line, Tumor
• Coordination Complexes (chemical synthesis, chemistry, metabolism)
• Energy Transfer
• Female
• Green Fluorescent Proteins (genetics, metabolism)
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Microscopy, Fluorescence
• Neoplasms (metabolism, pathology)
• Nitroimidazoles (chemistry)
• Optical Imaging
• Oxygen (metabolism)
• Ruthenium (chemistry)
• Transplantation, Heterologous