Bergenin Plays an Anti-Inflammatory Role via the Modulation of MAPK and NF-κB Signaling Pathways in a Mouse Model of LPS-Induced Mastitis.

Abstract

Mastitis is a major disease in humans and other animals and is characterized by mammary gland inflammation. It is a major disease of the dairy industry. Bergenin is an active constituent of the plants of genus Bergenia. Research indicates that bergenin has multiple biological activities, including anti-inflammatory and immunomodulatory properties. The objective of this study was to evaluate the protective effects and mechanism of bergenin on the mammary glands during lipopolysaccharide (LPS)-induced mastitis. In this study, mice were treated with LPS to induce mammary gland mastitis as a model for the disease. Bergenin treatment was initiated after LPS stimulation for 24 h. The results indicated that bergenin attenuated inflammatory cell infiltration and decreased the concentration of NO, TNF-α, IL-1β, and IL-6, which were increased in LPS-induced mouse mastitis. Furthermore, bergenin downregulated the phosphorylation of nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPK) signaling pathway proteins in mammary glands with mastitis. In conclusion, bergenin reduced the expression of NO, TNF-α, IL-1β, and IL-6 proinflammatory cytokines by inhibiting the activation of the NF-κB and MAPKs signaling pathways, and it may represent a novel treatment strategy for mastitis.

• Authors: Xue-jiao Gao, Meng-yao Guo, Ze-cai Zhang, Tian-cheng Wang, Yong-guo Cao, Nai-sheng Zhang
• Journal: Inflammation (Inflammation) Vol. 38 Issue 3 Pg. 1142-50 ( 2015) ISSN: 1573-2576 [Electronic] United States
• PMID: 25487780 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Benzopyrans
• IL1B protein, mouse
• Interleukin-1beta
• Interleukin-6
• Lipopolysaccharides
• NF-kappa B
• Plant Extracts
• Tumor Necrosis Factor-alpha
• Nitric Oxide
• Mitogen-Activated Protein Kinases
• bergenin

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Benzopyrans (therapeutic use)
• Disease Models, Animal
• Female
• Inflammation (drug therapy)
• Interleukin-1beta (metabolism)
• Interleukin-6 (metabolism)
• Lipopolysaccharides
• MAP Kinase Signaling System (drug effects)
• Mammary Glands, Animal (immunology, pathology)
• Mastitis (drug therapy, immunology, pathology)
• Mice
• Mice, Inbred BALB C
• Mitogen-Activated Protein Kinases (metabolism)
• NF-kappa B (metabolism)
• Nitric Oxide (metabolism)
• Phosphorylation (drug effects)
• Plant Extracts (therapeutic use)
• Tumor Necrosis Factor-alpha (metabolism)