VaxCelerate II: rapid development of a self-assembling vaccine for Lassa fever.

Abstract

Development of effective vaccines against emerging infectious diseases (EID) can take as much or more than a decade to progress from pathogen isolation/identification to clinical approval. As a result, conventional approaches fail to produce field-ready vaccines before the EID has spread extensively. Lassa is a prototypical emerging infectious disease endemic to West Africa for which no successful vaccine is available. We established the VaxCelerate Consortium to address the need for more rapid vaccine development by creating a platform capable of generating and pre-clinically testing a new vaccine against specific pathogen targets in less than 120 d A self-assembling vaccine is at the core of the approach. It consists of a fusion protein composed of the immunostimulatory Mycobacterium tuberculosis heat shock protein 70 (MtbHSP70) and the biotin binding protein, avidin. Mixing the resulting protein (MAV) with biotinylated pathogen-specific immunogenic peptides yields a self-assembled vaccine (SAV). To meet the time constraint imposed on this project, we used a distributed R&D model involving experts in the fields of protein engineering and production, bioinformatics, peptide synthesis/design and GMP/GLP manufacturing and testing standards. SAV immunogenicity was first tested using H1N1 influenza specific peptides and the entire VaxCelerate process was then tested in a mock live-fire exercise targeting Lassa fever virus. We demonstrated that the Lassa fever vaccine induced significantly increased class II peptide specific interferon-γ CD4(+) T cell responses in HLA-DR3 transgenic mice compared to peptide or MAV alone controls. We thereby demonstrated that our SAV in combination with a distributed development model may facilitate accelerated regulatory review by using an identical design for each vaccine and by applying safety and efficacy assessment tools that are more relevant to human vaccine responses than current animal models.

• Authors: Pierre Leblanc, Leonard Moise, Cybelle Luza, Kanawat Chantaralawan, Lynchy Lezeau, Jianping Yuan, Mary Field, Daniel Richer, Christine Boyle, William D Martin, Jordan B Fishman, Eric A Berg, David Baker, Brandon Zeigler, Dale E Mais, William Taylor, Russell Coleman, H Shaw Warren, Jeffrey A Gelfand, Anne S De Groot, Timothy Brauns, Mark C Poznansky
• Journal: Human vaccines & immunotherapeutics (Hum Vaccin Immunother) Vol. 10 Issue 10 Pg. 3022-38 ( 2014) ISSN: 2164-554X [Electronic] United States
• PMID: 25483693 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Review)

Chemical References

• Bacterial Proteins
• HLA-DR3 Antigen
• HSP70 Heat-Shock Proteins
• HSP70 protein, Mycobacterium tuberculosis
• Recombinant Fusion Proteins
• Viral Vaccines
• Avidin
• Interferon-gamma
• Ovalbumin

Topics

• Animals
• Avidin (immunology, therapeutic use)
• Bacterial Proteins (immunology, therapeutic use)
• CD4-Positive T-Lymphocytes (immunology)
• Communicable Diseases, Emerging (prevention & control)
• Female
• HLA-DR3 Antigen (genetics)
• HSP70 Heat-Shock Proteins (immunology, therapeutic use)
• Influenza A Virus, H1N1 Subtype (immunology)
• Interferon-gamma (immunology)
• Lassa Fever (immunology, prevention & control)
• Lassa virus (immunology)
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Mycobacterium tuberculosis (immunology)
• Ovalbumin (immunology)
• Protein Engineering
• Recombinant Fusion Proteins (immunology, therapeutic use)
• Viral Vaccines (immunology, therapeutic use)