HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

ShRNA-mediated silencing of the ubiquitin-specific protease 22 gene restrained cell progression and affected the Akt pathway in nasopharyngeal carcinoma.

Abstract
Ubiquitin-specific protease 22 (USP22) is closely related with poor prognosis of cancer patients. However, the role of USP22 expression in nasopharyngeal carcinoma (NPC) has not been determined. The main aim of this study was to determine the role of USP22 in the pathologic processes of NPC. Immunohistochemistry (IHC), western blot (WB), and real-time polymerase chain reaction (RT-PCR) were used to measure the expression of USP22 in cell lines and tissues of NPC in comparison with expression in non-cancerous cells and tissues. USP22-specific short hairpin RNA (shRNA) was used to knock down USP22 expression in the NPC cell line CNE-1 and CNE-2. Furthermore, the impact of USP22 in cellular proliferation, growth, and cell cycle were detected respectively. WB was used to determine the role of USP22 in the AKT/GSK-3/Cyclin signaling pathway. The expression levels of USP22 were remarkably higher in NPC cell lines and tissues. With cell counting and the MTS assay, cellular growth and proliferation progression of USP22 knockdown cell line was shown to be effectively restrained. The USP22 silencing both in CNE-1 and CNE-2 cells caused them to accumulate in the G0/G1 phase of the cell cycle. USP22 knockdown was also found to modulate the AKT/GSK-3/Cyclin pathway, resulting in downregulation of p-AKT, p-GSK-3β, and cyclinD1. This study suggests that USP22 plays a critical regulatory role in the pathologic processes of NPC, and that it may be a potential biological treatment target in the future.
AuthorsYa-Jing Zhuang, Zhi-Wei Liao, Hong-Wei Yu, Xian-Lu Song, Yuan Liu, Xing-Yuan Shi, Xiao-Dan Lin, Tong-Chong Zhou
JournalCancer biology & therapy (Cancer Biol Ther) Vol. 16 Issue 1 Pg. 88-96 ( 2015) ISSN: 1555-8576 [Electronic] United States
PMID25482932 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Carcinogens
  • RNA, Messenger
  • RNA, Small Interfering
  • GSK3B protein, human
  • Glycogen Synthase Kinase 3 beta
  • Proto-Oncogene Proteins c-akt
  • Glycogen Synthase Kinase 3
  • Thiolester Hydrolases
  • Ubiquitin Thiolesterase
  • Usp22 protein, human
Topics
  • Carcinogens
  • Carcinoma
  • Cell Cycle Checkpoints (genetics)
  • Cell Line, Tumor
  • Cell Survival (genetics)
  • Disease Progression
  • Gene Expression
  • Gene Silencing
  • Glycogen Synthase Kinase 3 (metabolism)
  • Glycogen Synthase Kinase 3 beta
  • Humans
  • Mucous Membrane (metabolism)
  • Nasopharyngeal Carcinoma
  • Nasopharyngeal Neoplasms (genetics, metabolism, pathology)
  • Proto-Oncogene Proteins c-akt (metabolism)
  • RNA, Messenger (genetics, metabolism)
  • RNA, Small Interfering (genetics)
  • Signal Transduction
  • Thiolester Hydrolases (genetics)
  • Ubiquitin Thiolesterase

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: