Abstract |
The PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK) is highly expressed in many types of tumors. However, its role in lung adenocarcinoma remains elusive. The aims of this study were to investigate the correlation between PBK/TOPK and mutant p53 in lung adenocarcinoma and to evaluate the effect of PBK/TOPK on cell proliferation and viability. Expression of PBK/TOPK and mutant p53 was detected in 127 cases of lung adenocarcinoma and was examined in the A549, GLC-82, and H358 lung adenocarcinoma cell lines by immunohistochemistry staining and Western blot assay. When PBK/TOPK expression was down-regulated by TOPK-specific siRNA in the A549 and GLC-82 lines, the effects of PBK/TOPK on cell proliferation, viability, and mutant p53 expression were evaluated. Expression of PBK/TOPK correlated positively with mutant p53 in both tumor tissues and cell lines. Kaplan-Meier survival analysis demonstrated that PBK/TOPK, mutant p53, lymph node metastasis, distant metastasis, high TNM stage, and poor tumor differentiation were associated with a poor prognosis. Cox multivariate analysis showed that PBK/TOPK, mutant p53, lymph node metastasis, and distant metastasis could each serve as an independent prognostic factor. After down-regulation of PBK/TOPK in the A549 and GLC-82 cell lines, mutant p53 expression was decreased, and cell proliferation and viability were significantly inhibited. Therefore, our results suggest that PBK/TOPK correlates with mutant p53 and affects cell proliferation and viability as well as prognosis in lung adenocarcinoma.
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Authors | Bin Lei, Wenjuan Qi, Yunfei Zhao, Yumei Li, Shuguang Liu, Xiaoyan Xu, Chen Zhi, Liyan Wan, Hong Shen |
Journal | Human pathology
(Hum Pathol)
Vol. 46
Issue 2
Pg. 217-24
(Feb 2015)
ISSN: 1532-8392 [Electronic] United States |
PMID | 25466965
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Inc. All rights reserved. |
Chemical References |
- TP53 protein, human
- Tumor Suppressor Protein p53
- Mitogen-Activated Protein Kinase Kinases
- PDZ-binding kinase
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Topics |
- Adenocarcinoma
(diagnosis, genetics, metabolism)
- Adenocarcinoma of Lung
- Adult
- Aged
- Aged, 80 and over
- Cell Cycle
(physiology)
- Cell Differentiation
(genetics, physiology)
- Cell Line, Tumor
- Cell Proliferation
(physiology)
- Cell Survival
- Female
- Humans
- Lung Neoplasms
(diagnosis, genetics, metabolism)
- Male
- Middle Aged
- Mitogen-Activated Protein Kinase Kinases
(genetics, metabolism)
- Neoplasm Staging
- Prognosis
- Tumor Suppressor Protein p53
(genetics, metabolism)
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