Monotropein exerts protective effects against IL-1β-induced apoptosis and catabolic responses on osteoarthritis chondrocytes.

Abstract

Osteoarthritis, characterized by a loss of articular cartilage accompanied with inflammation, is the most common age-associated degenerative disease. Monotropein, an iridoids glycoside isolated from the roots of Morinda officinalis How, has been demonstrated to exhibit anti-inflammatory activity. In the present study, monotropein was firstly to exhibit cartilage protective activity by down regulating the pro-inflammatory cytokines in the knee synovial fluid in vivo. The anti-apoptotic and anti-catabolic effects of monotropein on rat OA chondrocytes treated by IL-1β were investigated in vitro. In cultured chondrocytes, monotropein attenuated apoptosis in a dose-dependent manner in response to IL-1β stimulation. Moreover, treatment with monotropein, the expressions of MMP-3 and MMP-13 were significantly decreased, the expression of COL2A1 was increased. Taken together, these findings suggested that monotropein exerted anti-apoptosis and anti-catabolic activity in chondrocytes, which might support its possible therapeutic role in OA.

• Authors: Feng Wang, Longhuo Wu, Linfu Li, Siyi Chen
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 23 Issue 2 Pg. 575-80 (Dec 2014) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 25466264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Interleukin-1beta
• Iridoids
• monotropein
• Matrix Metalloproteinase 13
• Matrix Metalloproteinase 3

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Survival (drug effects)
• Cells, Cultured
• Chondrocytes (drug effects, immunology, pathology)
• Disease Models, Animal
• Interleukin-1beta (genetics, immunology)
• Iridoids (isolation & purification, pharmacology)
• Matrix Metalloproteinase 13 (genetics)
• Matrix Metalloproteinase 3 (genetics)
• Morinda (chemistry)
• Osteoarthritis (immunology, pathology)
• Plant Roots (chemistry)
• Primary Cell Culture
• Rats, Inbred Strains