E-selectin expression and BRAF status in papillary thyroid carcinomas: Correlation with clinicopathologic features.

Abstract

BACKGROUND:

Cell adhesion molecules, represented by the immunoglobulin family and selectins, play an important role in the progression of cancer. A correlation between selectins and tumor aggressiveness has been demonstrated in several reports.

METHODS:

Eighty-eight patients (mean age, 41.0 ± 14 years) with papillary thyroid carcinoma (conventional variant and sized approximately 20 mm) were divided in 2 groups: 41 with encapsulated tumors and 47 with tumors with extrathyroidal extension. E-selectin expression was evaluated by immunohistochemical staining and semiquantitative real-time reverse-transcription polymerase chain reaction and normalized by calculating the z-score (positive: value above the population mean; negative: below the mean).

RESULTS:

Lymph node metastasis (LNM) was found in 2 of 41 encapsulated tumors (4.8%) and in 19 of 47 tumors (40.4%) with extrathyroidal extension. BRAF mutation was present in 21 encapsulated tumors (51.2%) and in 31 tumors with extrathyroidal extension (65.9%). The mean E-selectin z-score was -0.32 for encapsulated tumors and 0.28 for tumors with extrathyroidal extension. E-selectin expression correlates with neoplastic infiltration (P = .04), the American Joint Commission on Cancer stage (P = .02), and BRAF mutation (P = .03).

CONCLUSION:

E-selectin overexpression in association with BRAF mutation status could promote a more aggressive phenotype in papillary thyroid carcinoma.

• Authors: Paolo Miccoli, Liborio Torregrossa, Nicla Borrelli, Gabriele Materazzi, Andrea Cacciato Insilla, Mario Miccoli, Fulvio Basolo
• Journal: Surgery (Surgery) Vol. 156 Issue 6 Pg. 1550-7; discussion 1557-8 (Dec 2014) ISSN: 1532-7361 [Electronic] United States
• PMID: 25456953 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• E-Selectin
• BRAF protein, human
• Proto-Oncogene Proteins B-raf

Topics

• Adult
• Aged
• Biopsy, Needle
• Carcinoma (genetics, secondary)
• Carcinoma, Papillary
• Cohort Studies
• Disease Progression
• E-Selectin (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Lymph Nodes (pathology)
• Lymphatic Metastasis
• Male
• Middle Aged
• Mutation
• Proto-Oncogene Proteins B-raf (genetics)
• Real-Time Polymerase Chain Reaction
• Retrospective Studies
• Sensitivity and Specificity
• Thyroid Cancer, Papillary
• Thyroid Neoplasms (genetics, pathology, secondary)
• Tissue Embedding
• Young Adult