Quantification of 3-n-butylphthalide in beagle plasma samples by supercritical fluid chromatography with triple quadruple mass spectrometry and its application to an oral bioavailability study.

A high-throughput, rapid, sensitive, environmentally friendly, and economical supercritical fluid chromatography with triple quadruple mass spectrometry method was established and validated for the first time to determine a cerebral stroke treatment drug named 3-n-butylphthalide in dog plasma. Plasma samples were prepared by protein precipitation with methanol and the analytes were eluted on an ACQUITY UPC(2TM) HSS-C(18) SB column (3 × 100 mm, 1.8 μm) maintained at 50°C. The mobile phase comprised supercritical carbon dioxide/methanol (90:10, v/v) at a flow rate of 1.5 mL/min, the compensation solvent was methanol at a flow rate of 0.2 mL/min and the total run time was 1.5 min per sample. The detection was carried out on a tandem mass spectrometer with an electrospray ionization source. Calibration curves were linear over the concentration range of 1.02-1021.00 ng/mL (r(2) ≥ 0.993) with the lower limit of quantification of 1.02 ng/mL. The intra- and inter-day precision values were below 15% and the accuracy was from 97.90 to 103.70% at all quality control levels. The method was suitable for a pharmacokinetic study of 3-n-butylphthalide in beagle dogs.
AuthorsYun Li, Longshan Zhao, Xiaoting Li, Bei Guo, Juanhang Zhao, Xianglin Wang, Tianhong Zhang
JournalJournal of separation science (J Sep Sci) Vol. 38 Issue 4 Pg. 697-702 (Feb 2015) ISSN: 1615-9314 [Electronic] Germany
PMID25446715 (Publication Type: Evaluation Studies, Journal Article)
Copyright© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Chemical References
  • Benzofurans
  • Drugs, Chinese Herbal
  • 3-n-butylphthalide
  • Animals
  • Benzofurans (administration & dosage, blood, pharmacokinetics)
  • Biological Availability
  • Chromatography, Supercritical Fluid (methods)
  • Dogs
  • Drugs, Chinese Herbal (administration & dosage, analysis, pharmacokinetics)
  • Male
  • Mass Spectrometry (methods)

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