Abstract |
A high-throughput, rapid, sensitive, environmentally friendly, and economical supercritical fluid chromatography with triple quadruple mass spectrometry method was established and validated for the first time to determine a cerebral stroke treatment drug named 3-n-butylphthalide in dog plasma. Plasma samples were prepared by protein precipitation with methanol and the analytes were eluted on an ACQUITY UPC(2TM) HSS-C(18) SB column (3 × 100 mm, 1.8 μm) maintained at 50°C. The mobile phase comprised supercritical carbon dioxide/ methanol (90:10, v/v) at a flow rate of 1.5 mL/min, the compensation solvent was methanol at a flow rate of 0.2 mL/min and the total run time was 1.5 min per sample. The detection was carried out on a tandem mass spectrometer with an electrospray ionization source. Calibration curves were linear over the concentration range of 1.02-1021.00 ng/mL (r(2) ≥ 0.993) with the lower limit of quantification of 1.02 ng/mL. The intra- and inter-day precision values were below 15% and the accuracy was from 97.90 to 103.70% at all quality control levels. The method was suitable for a pharmacokinetic study of 3-n-butylphthalide in beagle dogs.
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Authors | Yun Li, Longshan Zhao, Xiaoting Li, Bei Guo, Juanhang Zhao, Xianglin Wang, Tianhong Zhang |
Journal | Journal of separation science
(J Sep Sci)
Vol. 38
Issue 4
Pg. 697-702
(Feb 2015)
ISSN: 1615-9314 [Electronic] Germany |
PMID | 25446715
(Publication Type: Evaluation Study, Journal Article)
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Copyright | © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- Benzofurans
- Drugs, Chinese Herbal
- 3-n-butylphthalide
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Topics |
- Animals
- Benzofurans
(administration & dosage, blood, pharmacokinetics)
- Biological Availability
- Chromatography, Supercritical Fluid
(methods)
- Dogs
- Drugs, Chinese Herbal
(administration & dosage, analysis, pharmacokinetics)
- Male
- Mass Spectrometry
(methods)
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