Starving/re-feeding processes induce metabolic modifications in thick-lipped grey mullet (Chelon labrosus, Risso 1827).

Abstract

The effects of starvation and re-feeding on metabolites and tissue composition, GH/IGF-I axis, and digestive enzyme activities in juvenile thick-lipped grey mullet (Chelon labrosus) were evaluated. Fish were divided into three feeding groups (n=72, 82.00±4.09 g initial body mass). The control group was fed 1% of their body mass once a day throughout the experiment with commercial pellets. The other two groups were deprived of feed for 21 days (starved), or re-fed for 7 days after 14 days of food deprivation (re-fed). Full-length cDNAs from pituitary GH and hepatic IGF-I were cloned by screening a cDNA library or by PCR techniques. Furthermore, changes in their mRNA expressions were assessed by real time PCR in specimens maintained under the different feeding patterns. Results showed a negative growth in starved and re-feeding groups. Starvation induced a significant increase in plasma triglycerides as well as a decrease in liver glucose and glycogen. Re-feeding increased plasma glucose, lactate and protein, as well as liver glucose and glycogen. In addition, starvation significantly increased pituitary GH expression, while re-feeding down-regulated it. No significant changes were observed in hepatic IGF-I expression in any dietary treatment. Digestive enzyme activities were not significantly affected either by starvation or by re-feeding. The results of the present work suggest that juveniles of the thick-lipped grey mullet may easily adjust their metabolism under situations characterized by a short-term starvation and re-feeding.

• Authors: I M Pujante, J A Martos-Sitcha, F J Moyano, I Ruiz-Jarabo, G Martínez-Rodríguez, J M Mancera
• Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (Comp Biochem Physiol B Biochem Mol Biol) Vol. 180 Pg. 57-67 (Feb 2015) ISSN: 1879-1107 [Electronic] England
• PMID: 25446605 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• RNA, Messenger
• Insulin-Like Growth Factor I
• Growth Hormone

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Digestive System (enzymology)
• Energy Metabolism
• Growth Hormone (metabolism)
• Insulin-Like Growth Factor I (metabolism)
• Molecular Sequence Data
• Organ Specificity
• RNA, Messenger (metabolism)
• Smegmamorpha (metabolism)
• Starvation