Abstract | UNLABELLED: MATERIALS AND METHODS: For this study, patients with stages I-III breast cancers and adjacent noncancerous tissues were prospectively accrued and analyzed. We employed semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis to determine the expression levels of TSHR in normal and human breast cancer tissues. RESULTS: The results indicated that a significant increase in TSHR expression was observed in tumor tissues compared to normal breast tissues. RT-PCR analysis of OCT4 and IPP also revealed a significant increase in breast tumor tissues over the controls. CONCLUSIONS: To our knowledge, this is the first report demonstrating expression of TSHR and IPP in normal breast and breast tumors. The expression of TSHR, IPP, and OCT4 increased in the human breast tumor samples over the noncancer tissues. However, further studies are needed to establish an unequivocal role for TSHR in breast tumor progression.
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Authors | Vijayakumar Govindaraj, Nirmala Singh Yaduvanshi, Harish Krishnamachar, A Jagannadha Rao |
Journal | Hormone molecular biology and clinical investigation
(Horm Mol Biol Clin Investig)
Vol. 9
Issue 3
Pg. 173-8
(Apr 2012)
ISSN: 1868-1883 [Print] Germany |
PMID | 25436706
(Publication Type: Journal Article)
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