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Paeoniflorin attenuates allergic inflammation in asthmatic mice.

Abstract
Paeoniflorin (PF), one of the major active ingredients of Chinese peony, has demonstrated anti-inflammatory and immunoregulatory effects. However, it has remained unclear whether PF treatment can inhibit allergic inflammation in asthma. In this study, we evaluated the effects of PF on pulmonary function and airway inflammation in asthmatic mice. The allergic asthma models were established in BALB/c mice. The mice were sensitized and challenged with ovalbumin. Airway hyperresponsiveness was detected by direct airway resistance analysis. Lung tissues were examined for inflammatory cell infiltration. IL-5, IL-13, IL-17, and eotaxin in bronchoalveolar lavage fluid (BALF) and their mRNA expression in lung tissue were examined by ELISA and realtime PCR, respectively. The total IgE level in serum was measured by ELISA. The protein expression of p-ERK and p-JNK was detected by western blot. Our data showed that PF oral administration significantly reduced airway hyperresponsiveness to aerosolized methacholine and decreased IL-5, IL-13, IL-17 and eotaxin levels in the BALF, and decreased IgE level in the serum. Histological studies showed that PF administration markedly decreased inflammatory infiltration. Similarly, treatment with PF significantly inhibited IL-5, IL-13, IL-17 and eotaxin mRNA expression in lung tissues. The protein expression levels of p-ERK and p-JNK were substantially decreased after oral administration of PF. In summary, PF displayed anti-inflammatory effects in the OVA-induced asthmatic model by decreasing the expression of IL-5, IL-13, IL-17 and eotaxin. These effects were mediated at least partially by inhibiting the activation of MAPK pathway.
AuthorsJing Sun, Jinfeng Wu, Changqing Xu, Qingli Luo, Bei Li, Jingcheng Dong
JournalInternational immunopharmacology (Int Immunopharmacol) Vol. 24 Issue 1 Pg. 88-94 (Jan 2015) ISSN: 1878-1705 [Electronic] Netherlands
PMID25433342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier B.V. All rights reserved.
Chemical References
  • Anti-Inflammatory Agents, Non-Steroidal
  • Ccl11 protein, mouse
  • Chemokine CCL11
  • Cytokines
  • Glucosides
  • Inflammation Mediators
  • Monoterpenes
  • peoniflorin
Topics
  • Administration, Oral
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, adverse effects)
  • Asthma (drug therapy, immunology)
  • Bronchial Hyperreactivity (drug therapy, immunology)
  • Bronchial Provocation Tests
  • Chemokine CCL11 (genetics, metabolism)
  • Cytokines (genetics, metabolism)
  • Disease Models, Animal
  • Down-Regulation (drug effects)
  • Female
  • Glucosides (administration & dosage, adverse effects)
  • Inflammation Mediators (metabolism)
  • Lung (drug effects, immunology)
  • Mice
  • Mice, Inbred BALB C
  • Monoterpenes (administration & dosage, adverse effects)
  • Paeonia (immunology)

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