Abstract |
T cells are required to maintain the latency of chronic infection with Toxoplasma gondii in the brain. Here, we examined the role of non- glutamic acid- leucine- arginine CXC chemokine CXCL9 for T-cell recruitment to prevent reactivation of infection with T. gondii. Severe combined immunodeficient (SCID) mice were infected and treated with sulfadiazine to establish a chronic infection. Immune T cells from infected wild-type mice were transferred into the SCID mice in combination with treatment with anti-CXCL9 or control sera. Three days later, sulfadiazine was discontinued to initiate reactivation of infection. Numbers of CD4(+) and CD8(+) T cells isolated from the brains were markedly less in mice treated with anti-CXCL9 serum than in mice treated with control serum at 3 days after sulfadiazine discontinuation. Amounts of tachyzoite (acute stage form of T. gondii)-specific SAG1 mRNA and numbers of foci associated with tachyzoites were significantly greater in the former than the latter at 5 days after sulfadiazine discontinuation. An accumulation of CD3(+) T cells into the areas of tachyzoite growth was significantly less frequent in the SCID mice treated with anti-CXCL9 serum than in mice treated with control serum. These results indicate that CXCL9 is crucial for recruiting immune T cells into the brain and inducing an accumulation of the T cells into the areas where tachyzoites proliferate to prevent reactivation of chronic T. gondii infection.
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Authors | Eri Ochiai, Qila Sa, Morgan Brogli, Tomoya Kudo, Xisheng Wang, Jitender P Dubey, Yasuhiro Suzuki |
Journal | The American journal of pathology
(Am J Pathol)
Vol. 185
Issue 2
Pg. 314-24
(Feb 2015)
ISSN: 1525-2191 [Electronic] United States |
PMID | 25432064
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2015 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Chemokine CXCL9
- Cxcl9 protein, mouse
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Topics |
- Animals
- Brain
(immunology, parasitology, pathology)
- CD4-Positive T-Lymphocytes
(immunology, pathology)
- CD8-Positive T-Lymphocytes
(immunology, pathology)
- Cell Movement
(genetics, immunology)
- Cell Proliferation
- Chemokine CXCL9
(genetics, immunology)
- Chronic Disease
- Encephalitis
(genetics, immunology, parasitology, pathology)
- Mice
- Mice, Inbred BALB C
- Mice, SCID
- Toxoplasma
(immunology)
- Toxoplasmosis
(genetics, immunology, pathology)
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