HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

ROS inhibit autophagy by downregulating ULK1 mediated by the phosphorylation of p53 in selenite-treated NB4 cells.

Abstract
Reactive oxygen species (ROS) have an important role in regulating various cellular processes. Our previous study confirmed that selenite, an anti-tumour agent, triggered apoptosis through the production of ROS in multiple types of cancer cells. In this study, we discovered that ROS also inhibited protective autophagy by decreasing the expression of ULK1, an initiator of autophagy, in selenite-treated NB4 cells. Further experiments demonstrated that p-p53 (S392), a phosphorylation event promoted by p70S6K, bound to the promoter of ULK1 and modulated its expression. Experiments in a mouse tumour model with NB4 cells provided in vivo confirmation of the alterations in the p70S6K/p53/ULK1 axis. Collectively, our results show that ROS inhibited autophagy by downregulating the p70S6K/p53/ULK1 axis in selenite-treated NB4 cells.
AuthorsY Ci, K Shi, J An, Y Yang, K Hui, P Wu, L Shi, C Xu
JournalCell death & disease (Cell Death Dis) Vol. 5 Pg. e1542 (Nov 27 2014) ISSN: 2041-4889 [Electronic] England
PMID25429619 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Intracellular Signaling Peptides and Proteins
  • Reactive Oxygen Species
  • Tumor Suppressor Protein p53
  • Phosphoserine
  • Autophagy-Related Protein-1 Homolog
  • Protein Serine-Threonine Kinases
  • Ribosomal Protein S6 Kinases, 70-kDa
  • ULK1 protein, human
  • Selenious Acid
Topics
  • Animals
  • Apoptosis (drug effects)
  • Autophagy (drug effects)
  • Autophagy-Related Protein-1 Homolog
  • Cell Line, Tumor
  • Down-Regulation (drug effects)
  • Humans
  • Intracellular Signaling Peptides and Proteins (metabolism)
  • Mice
  • Models, Biological
  • Phosphorylation (drug effects)
  • Phosphoserine (metabolism)
  • Protein Serine-Threonine Kinases (metabolism)
  • Reactive Oxygen Species (metabolism)
  • Ribosomal Protein S6 Kinases, 70-kDa (metabolism)
  • Selenious Acid (pharmacology)
  • Signal Transduction (drug effects)
  • Tumor Suppressor Protein p53 (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: