The protective effects of dexmedetomidine on hepatic ischemia reperfusion injury.

Abstract

OBJECTIVE:

The aim of this study was to evaluate the effect of dexmedetomidine (100 µg/kg-ip) on liver ischemia and reperfusion (I/R) in rats.

METHODS:

Twenty-four Wistar Albino rats were separated into three groups as control (C), ischemia-reperfusion injury (I/R) and dexmedetomidine group (I/R-D). Ischemia was induced with portal clampage for 45 minutes and reperfusion period was 45 minutes after declampage. Group I/R-D was received dexmedetomidine 100 µg/kg i.p. 30 min before portal clampage. Thiobarbutiric Acid-Reactive Substances (TBARS), glutathioneS-transferase (GST), superoxide dismutase (SOD), Catalase (CAT), and Paraoxonase 1 (PON-1) were investigated in blood samples. Also HSP60 and p53-positive hepatocytes were counted under ImageJ image analysis program.

RESULTS:

All parameters, except GST levels, were significant between the groups (p < 0.05). Although HSP60 expression was significantly increased between I/R, I/R-D and C groups there were no significant differences between I/R-D and C (p = 0.443). On the other hand, p53 expression was also significantly increased between I/R, I/R-D and C groups At the same time, there were no significant differences between I/R-D and C groups (p = 0.354).

CONCLUSION:

All the results suggest that dexmedetomidine has beneficial effects on liver ischemia/reperfusion stress (Tab. 1, Fig. 2, Ref. 49).

• Authors: A Kucuk, F Yaylak, A Cavunt-Bayraktar, M Tosun, M Arslan, F M Comu, M Kavutcu
• Journal: Bratislavske lekarske listy (Bratisl Lek Listy) Vol. 115 Issue 11 Pg. 680-4 ( 2014) ISSN: 0006-9248 [Print] Slovakia
• PMID: 25428535 (Publication Type: Journal Article)

Chemical References

• Protective Agents
• Dexmedetomidine

Topics

• Animals
• Dexmedetomidine (pharmacology)
• Ischemia (drug therapy)
• Ischemic Preconditioning (methods)
• Liver (blood supply)
• Liver Diseases (drug therapy, prevention & control)
• Protective Agents (pharmacology)
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, prevention & control)