Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats.

Abstract

OBJECTIVE:

To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction (清瘟败毒饮, QBD) from the view of holistic biology.

METHODS:

The rats were randomly divided into a normal conrol group, a lipopolysaccharide (LPS) group, the low- and high-dose QBD groups, and a dexamethasone (DXM) group. NR8383 cells were treated with culture fluid containing 6% serum from rats of each group respectively. Inflammatory mediators were detected by reverse transcription polymerase chain reaction (RT-PCR), Western blotting hybridization, enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) gene array and antibody array.

RESULTS:

It is showed that the levels of interleukin (IL)-1α, IL-4 and IL-12 were enhanced in the low-dose QBD group; levels of IL-1α, IL-12 and IL-18 were augmented in the high-dose QBD group, compared with the LPS group after ELISA detection. Western blot showed that IL-1β and tumor necrosis factor (TNF)-α expression of the control group were lower than other groups. IL-1β level of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after 24 h than that of the control group (P<0.01). Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array. Up-regulation genes were 22 in both the LPS group and the low-dose QBD group, among which 16 up-regulating genes were the same. In these 16 genes, the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group, 4 were similar to and 3 were more. Twenty-nine main cytokines were inspected by rat cytokine antibody array. Intergroup gray value differences were found in 7 expressed cytokines. The levels of these 7 cytokines in the low-dose QBD group were all lower than those in the the LPS group.

CONCLUSIONS:

QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.

• Authors: Zheng-miao Yu, Zhi-hui Liu, Jing Chen, Qiang Zeng
• Journal: Chinese journal of integrative medicine (Chin J Integr Med) Vol. 20 Issue 12 Pg. 934-43 (Dec 2014) ISSN: 1672-0415 [Print] China
• PMID: 25428340 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Drugs, Chinese Herbal
• Inflammation Mediators
• qingwen baidu

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Blotting, Western
• Cell Line
• Cytokines (metabolism)
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Electrophoresis, Agar Gel
• Inflammation Mediators (metabolism)
• Lung (drug effects, metabolism, pathology)
• Male
• Nucleic Acid Denaturation
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Sepsis (drug therapy, pathology)