Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.

Abstract

PROBLEM:

The chemokine IL-8 recruits neutrophils to sites of infection, including the endometrium of the bovine uterus. However, quantification of bovine IL-8 often yields lower concentrations than for other species, which may reflect impaired innate immune responses by bovine cells or inaccurate measurement of IL-8 using the current human IL-8 ELISA method.

METHOD OF STUDY:

An ELISA was developed and validated for detection of bovine IL-8. Utility of the assay was tested by measuring the response of bovine endometrium and cells to bacteria and pathogen-associated molecular patterns.

RESULTS:

The developed ELISA detected 62.5-2000 pg/mL IL-8, with minimal cross-reactivity to other inflammatory mediators. Concentrations of bovine IL-8 were measured more accurately by the bovine than human IL-8 ELISA. Bovine endometrial IL-8 responses to pathogen-associated molecules were quantitatively similar to other species.

CONCLUSION:

A bovine-specific IL-8 ELISA was developed, which accurately measured IL-8 secretion from endometrial cells.

• Authors: James G Cronin, Rebecca Hodges, Sara Pedersen, Iain M Sheldon
• Journal: American journal of reproductive immunology (New York, N.Y. : 1989) (Am J Reprod Immunol) Vol. 73 Issue 4 Pg. 372-82 (Apr 2015) ISSN: 1600-0897 [Electronic] Denmark
• PMID: 25427847 (Publication Type: Journal Article)
• Copyright: © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Interleukin-8

Topics

• Animals
• Cattle
• Endometrium (immunology, microbiology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Genitalia, Female (immunology, microbiology)
• Interleukin-8 (immunology)
• Reproductive Tract Infections (immunology)