[Experimental study on effect of burn on brown adipose tissue in mice].

Abstract

OBJECTIVE:

To investigate the effect of burn on brown adipose tissue (BAT) in BALB/c mice.

METHODS:

Forty 3-4 months old male BALB/c mice with initial body weight of (20 ± 3) g were randomly divided into control group and burn group (n = 20). BALB/c mice in burn group were subjected to a 30% total body surface area (TBSA) full-thickness thermal injury. BALB/c mice in control group were not treated. The body weight and temperature were observed before and after burn. At 7 days after burn, morphological changes of white adipose tissue (WAT) and BAT were observed, the gene and protein expressions of uncoupling protein 1 (UCP-1) were detected.

RESULTS:

There was no significant difference in the body weight and body temperature before burn (P > 0.05). At 1, 2, 3, and 4 weeks after burn, the body weight was significantly lower in burn group than in control group (P < 0.05). At 1, 2, 3, and 7 days after burn, the body temperature was significantly higher in burn group than in control group (P < 0.05). At 7 days after burn, the weight of WAT was significantly reduced, and the weight of BAT was significantly increased in burn group (P < 0.05); WAT and BAT cells became smaller, cell number increased, the cytoplasm and mitochondria appeared as compact. The UCP-1 gene and protein expressions of burn group were significantly higher than those of control group (P < 0.05).

CONCLUSION:

BAT plays an important role in burn-induced hypermetabolism.

• Authors: Shubo Zhuang, Jiake Chai, Yonghui Yu, Huinan Yin, Lingying Liu, Jun Fan, Yihe Wang, Yusen Hou
• Journal: Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery (Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi) Vol. 28 Issue 8 Pg. 988-92 (Aug 2014) ISSN: 1002-1892 [Print] China
• PMID: 25417314 (Publication Type: Journal Article)

Chemical References

• Ion Channels
• Mitochondrial Proteins
• Ucp1 protein, mouse
• Uncoupling Protein 1

Topics

• Adipose Tissue, Brown (physiology)
• Adipose Tissue, White (physiology)
• Animals
• Body Temperature
• Body Weight
• Burns (complications)
• Ion Channels (genetics, metabolism)
• Male
• Mice
• Mice, Inbred BALB C
• Mitochondrial Proteins (genetics, metabolism)
• Temperature
• Uncoupling Protein 1