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Hyperbaric oxygenation treatment alleviates CCI-induced neuropathic pain and decreases spinal apoptosis.

AbstractBACKGROUND:
Increased apoptotic changes in the spinal cord may be responsible for the development of chronic constriction injury (CCI)-induced neuropathic pain. We previously reported the beneficial effect of hyperbaric oxygen (HBO) in the treatment of CCI-induced neuropathic pain. In this study, we tested our hypotheses that HBO may achieve its beneficial effect by inhibiting CCI-induced proapoptosis gene expression and apoptosis in the spinal cord.
METHODS:
Male rats were randomized into: SHAM, CCI and CCI + HBO groups. Mechanical hyperalgesia was tested daily following surgery. CCI + HBO rats were treated with HBO for 1 h daily. At 3 days post-CCI, the expression of tumour necrosis factor (TNF)-α and caspase-3 genes was detected. At 7 days post-CCI, apoptotic cells in the spinal cord were detected.
RESULTS:
Three days post-CCI, mechanical allodynia had developed in the ipsilateral paw compared with SHAM animals. HBO significantly alleviated CCI-induced mechanical allodynia. In comparison with SHAM, CCI-induced neuropathic pain was associated with higher mRNA levels of TNF-α and caspase-3. HBO significantly decreased CCI-induced mRNA levels of TNF-α and caspase-3. CCI-induced neuropathic pain was also associated with more apoptotic cells in the spinal cord 7 days post-CCI. HBO significantly reduced CCI-induced apoptosis to the level of SHAM animals.
CONCLUSIONS:
Overly expressed proapoptosis genes, and subsequent increase in spinal apoptotic cells, seem to contribute to the development of CCI-induced neuropathic pain. The inhibitory role of HBO on spinal proapoptosis genes and apoptotic changes may contribute to its beneficial effect on CCI-induced neuropathic pain.
AuthorsQ Hu, L Fang, F Li, S Thomas, Z Yang
JournalEuropean journal of pain (London, England) (Eur J Pain) Vol. 19 Issue 7 Pg. 920-8 (Aug 2015) ISSN: 1532-2149 [Electronic] England
PMID25410300 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 European Pain Federation - EFIC®
Chemical References
  • Apoptosis Regulatory Proteins
  • Tumor Necrosis Factor-alpha
  • Caspase 3
Topics
  • Animals
  • Apoptosis (drug effects)
  • Apoptosis Regulatory Proteins (biosynthesis)
  • Behavior, Animal
  • Caspase 3 (biosynthesis, genetics)
  • Constriction, Pathologic (complications)
  • Hyperalgesia (psychology, therapy)
  • Hyperbaric Oxygenation
  • Male
  • Neuralgia (etiology, psychology, therapy)
  • Rats
  • Rats, Sprague-Dawley
  • Spinal Cord (pathology)
  • Tumor Necrosis Factor-alpha (biosynthesis, genetics)

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