Growth stimulatory effect of pancreatic spasmolytic polypeptide on cultured colon and breast tumor cells.

Abstract

The effects of a novel polypeptide, pancreatic spasmolytic polypeptide (PSP) on a colon carcinoma cell line (HCT 116) were examined. PSP stimulated the incorporation of [3H]thymidine into HCT 116 cells as well as cell proliferation in a dose-dependent manner. Maximal increase in [3H]thymidine incorporation of 50-60% occurred at 3-300 microM PSP. The VIP-mediated-increase in cAMP levels was reduced by PSP at greater than 1 microM concentrations. PSP is highly homologous to the estrogen-induced pS2 protein in MCF-7 breast cancer cells. We find that PSP also enhanced [3H]thymidine incorporation in MCF-7 cells. These findings indicate for the first time that PSP has growth stimulatory properties.

• Authors: N M Hoosein, L Thim, K H Jørgensen, M G Brattain
• Journal: FEBS letters (FEBS Lett) Vol. 247 Issue 2 Pg. 303-6 (Apr 24 1989) ISSN: 0014-5793 [Print] England
• PMID: 2541019 (Publication Type: Journal Article)

Chemical References

• Intercellular Signaling Peptides and Proteins
• Mucins
• Muscle Proteins
• Neuropeptides
• Parasympatholytics
• Peptides
• TFF3 protein, rat
• Tff2 protein, rat
• Trefoil Factor-2
• Trefoil Factor-3
• Vasoactive Intestinal Peptide
• pancreatic spasmolytic polypeptide
• DNA
• Cyclic AMP

Topics

• Animals
• Breast Neoplasms (metabolism, pathology)
• Cell Division (drug effects)
• Colonic Neoplasms (metabolism, pathology)
• Cyclic AMP (metabolism)
• DNA (biosynthesis)
• Humans
• Intercellular Signaling Peptides and Proteins
• Mucins
• Muscle Proteins
• Neuropeptides
• Parasympatholytics
• Peptides (pharmacology)
• Swine
• Trefoil Factor-2
• Trefoil Factor-3
• Tumor Cells, Cultured
• Vasoactive Intestinal Peptide (metabolism, pharmacology)