Ribosomal protein S15A augments human osteosarcoma cell proliferation in vitro.

Abstract

As a highly conserved housekeeping gene, the biological implications of ribosomal protein S15A (RPS15A) during various processes, including carcinogenesis, remain elusive. Herein, the authors reported that knockdown of RPS15A expression significantly inhibited human osteosarcoma U2OS cell proliferation and colony formation in vitro by using a lentivirus-mediated RNA interference (RNAi) system. Moreover, an excess accumulation of cells in the G0/G1 phase was observed in U2OS cells transduced with lentivirus targeting RPS15A, suggesting that the growth inhibition mediated by RPS15A knockdown in osteosarcoma cells was probably due to the induction of cell cycle arrest. Taken together, this study highlights the crucial role of RPS15A in promoting osteosarcoma cell proliferation, and provides a foundation for further study into the clinical potential of inhibition of RPS15A for the treatment of osteosarcoma.

• Authors: Chen Zhang, Ting Zhang, E Song, S W A Himaya, Xianzhen Chen, Longpo Zheng
• Journal: Cancer biotherapy & radiopharmaceuticals (Cancer Biother Radiopharm) Vol. 29 Issue 10 Pg. 451-6 (Dec 2014) ISSN: 1557-8852 [Electronic] United States
• PMID: 25409460 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)

Chemical References

• Ribosomal Proteins
• ribosomal protein S15a

Topics

• Bone Neoplasms (genetics)
• Cell Cycle Checkpoints (genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• G1 Phase (genetics)
• Humans
• Lentivirus (genetics)
• Osteosarcoma (genetics)
• RNA Interference (physiology)
• Resting Phase, Cell Cycle (genetics)
• Ribosomal Proteins (genetics)