Abstract | PURPOSE: PROCEDURES: The effects of docetaxel and cabazitaxel on viable tumor cell number were explored in four PC cell lines. Cellular uptake of [(18)F]FDG and [(18)F]FCH was compared with the effects measured using sulforhodamine B (SRB) assay, cell counting and colony formation assay (CFA), as proximators of viable tumor cell number. Agreement between uptake and cell numbers was assessed by Bland-Altman plots. RESULTS: [(18)F]FCH uptake in all PC cell lines significantly correlated to the cell numbers surviving the respective drug concentrations. Bland-Altman analysis showed that [(18)F]FDG uptake resulted in signal overestimation and higher variability after chemotherapy. CONCLUSIONS:
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Authors | Daniela E Oprea-Lager, Mitchell P van Kanten, Reindert J A van Moorselaar, Alfons J M van den Eertwegh, Peter M van de Ven, Irene V Bijnsdorp, Otto S Hoekstra, Albert A Geldof |
Journal | Molecular imaging and biology
(Mol Imaging Biol)
Vol. 17
Issue 3
Pg. 319-27
(Jun 2015)
ISSN: 1860-2002 [Electronic] United States |
PMID | 25407926
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antineoplastic Agents
- Fluorine Radioisotopes
- Radiopharmaceuticals
- Rhodamines
- Taxoids
- fluoromethylcholine
- Fluorodeoxyglucose F18
- Docetaxel
- lissamine rhodamine B
- cabazitaxel
- Choline
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Topics |
- Antineoplastic Agents
(therapeutic use)
- Cell Line, Tumor
(drug effects)
- Choline
(analogs & derivatives, chemistry)
- Docetaxel
- Drug Screening Assays, Antitumor
- Fluorine Radioisotopes
(chemistry)
- Fluorodeoxyglucose F18
(chemistry)
- Humans
- Inhibitory Concentration 50
- Male
- Prostatic Neoplasms
(diagnostic imaging, metabolism)
- Radionuclide Imaging
- Radiopharmaceuticals
(chemistry)
- Rhodamines
(chemistry)
- Taxoids
(therapeutic use)
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