[18F]fluoromethylcholine as a chemotherapy response read-out in prostate cancer cells.

Abstract

PURPOSE:

The objective of the present study is to determine whether uptake of [(18)F]fluoromethylcholine ([(18)F]FCH) in comparison with 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) accurately reflects chemotherapy efficacy at the tumor cell level in prostate cancer (PC).

PROCEDURES:

The effects of docetaxel and cabazitaxel on viable tumor cell number were explored in four PC cell lines. Cellular uptake of [(18)F]FDG and [(18)F]FCH was compared with the effects measured using sulforhodamine B (SRB) assay, cell counting and colony formation assay (CFA), as proximators of viable tumor cell number. Agreement between uptake and cell numbers was assessed by Bland-Altman plots.

RESULTS:

[(18)F]FCH uptake in all PC cell lines significantly correlated to the cell numbers surviving the respective drug concentrations. Bland-Altman analysis showed that [(18)F]FDG uptake resulted in signal overestimation and higher variability after chemotherapy.

CONCLUSIONS:

[(18)F]FCH uptake correlates well with viable tumor cell numbers remaining after docetaxel and cabazitaxel exposure. Radiolabeled choline is a potential response monitoring biomarker after chemotherapy for PC.

• Authors: Daniela E Oprea-Lager, Mitchell P van Kanten, Reindert J A van Moorselaar, Alfons J M van den Eertwegh, Peter M van de Ven, Irene V Bijnsdorp, Otto S Hoekstra, Albert A Geldof
• Journal: Molecular imaging and biology (Mol Imaging Biol) Vol. 17 Issue 3 Pg. 319-27 (Jun 2015) ISSN: 1860-2002 [Electronic] United States
• PMID: 25407926 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antineoplastic Agents
• Fluorine Radioisotopes
• Radiopharmaceuticals
• Rhodamines
• Taxoids
• fluoromethylcholine
• Fluorodeoxyglucose F18
• Docetaxel
• lissamine rhodamine B
• cabazitaxel
• Choline

Topics

• Antineoplastic Agents (therapeutic use)
• Cell Line, Tumor (drug effects)
• Choline (analogs & derivatives, chemistry)
• Docetaxel
• Drug Screening Assays, Antitumor
• Fluorine Radioisotopes (chemistry)
• Fluorodeoxyglucose F18 (chemistry)
• Humans
• Inhibitory Concentration 50
• Male
• Prostatic Neoplasms (diagnostic imaging, metabolism)
• Radionuclide Imaging
• Radiopharmaceuticals (chemistry)
• Rhodamines (chemistry)
• Taxoids (therapeutic use)