Abstract | BACKGROUND: METHODS: RESULTS: The tumor cells acquired round shapes, lost cytoplasmic expansions, formed clusters in suspension and decreased viability. Jari was almost 20 times more potent toxin than jara based on IC50 values and on morphological changes of the cells, also observed by scanning electron microscopy. Flow cytometry analysis showed 48.3% decrease in the proliferation rate of cells and 47.2% increase in apoptosis (jara) and necrosis (jari), following 1.2 μM jara and 0.1 μM jari treatments. Caspase-3 activity was increased whereas G0/G1 cell cycle phase was on the decline. Proliferative rate was assessed by staining with 5,6-carboxyfluoresceindiacetate succinimidyl ester, showing a significant decrease in proliferation at all concentrations of both toxins. CONCLUSIONS: In vivo treatment of the toxins was observed reduction in the incidence of nodules, and metastasis and antiproliferative inhibition capacity. This data strengthens the potential use jararhagin as an anti-neoplastic drug.
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Authors | Durvanei Augusto Maria, Manuela Garcia Laveli da Silva, Mario Cesar Correia Junior, Itamar Romano Garcia Ruiz |
Journal | BMC complementary and alternative medicine
(BMC Complement Altern Med)
Vol. 14
Pg. 446
(Nov 18 2014)
ISSN: 1472-6882 [Electronic] England |
PMID | 25407317
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Crotalid Venoms
- Platelet Aggregation Inhibitors
- Metalloproteases
- Caspase 3
- Metalloendopeptidases
- jararhagin
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology, therapeutic use)
- Apoptosis
(drug effects)
- Bothrops
- Caspase 3
(metabolism)
- Cell Adhesion
(drug effects)
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Crotalid Venoms
(isolation & purification, pharmacology, therapeutic use)
- Melanoma
(drug therapy, metabolism)
- Metalloendopeptidases
(isolation & purification, pharmacology, therapeutic use)
- Metalloproteases
(pharmacology, therapeutic use)
- Mice
- Platelet Aggregation
- Platelet Aggregation Inhibitors
(pharmacology, therapeutic use)
- Skin Neoplasms
(drug therapy, metabolism)
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