HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Analysis of protein-protein interactions in MCF-7 and MDA-MB-231 cell lines using phthalic acid chemical probes.

Abstract
Phthalates are a class of plasticizers that have been characterized as endocrine disrupters, and are associated with genital diseases, cardiotoxicity, hepatotoxicity, and nephrotoxicity in the GeneOntology gene/protein database. In this study, we synthesized phthalic acid chemical probes and demonstrated differing protein-protein interactions between MCF-7 cells and MDA-MB-231 breast cancer cell lines. Phthalic acid chemical probes were synthesized using silicon dioxide particle carriers, which were modified using the silanized linker 3-aminopropyl triethoxyslane (APTES). Incubation with cell lysates from breast cancer cell lines revealed interactions between phthalic acid and cellular proteins in MCF-7 and MDA-MB-231 cells. Subsequent proteomics analyses indicated 22 phthalic acid-binding proteins in both cell types, including heat shock cognate 71-kDa protein, ATP synthase subunit beta, and heat shock protein HSP 90-beta. In addition, 21 MCF-7-specific and 32 MDA-MB-231 specific phthalic acid-binding proteins were identified, including related proteasome proteins, heat shock 70-kDa protein, and NADPH dehydrogenase and ribosomal correlated proteins, ras-related proteins, and members of the heat shock protein family, respectively.
AuthorsShih-Shin Liang, Tsu-Nai Wang, Eing-Mei Tsai
JournalInternational journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 11 Pg. 20770-88 (Nov 13 2014) ISSN: 1422-0067 [Electronic] Switzerland
PMID25402641 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Phthalic Acids
  • Proteins
  • phthalic acid
Topics
  • Breast (metabolism)
  • Breast Neoplasms (metabolism)
  • Cell Line, Tumor
  • Female
  • Humans
  • MCF-7 Cells
  • Phthalic Acids (analysis, metabolism)
  • Protein Interaction Mapping (methods)
  • Protein Interaction Maps
  • Proteins (analysis, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: