Immunohistochemical studies of steroidogenic enzymes (aromatase, 17 alpha-hydroxylase and cholesterol side-chain cleavage cytochromes P-450) in sex cord-stromal tumors of the ovary.

Abstract

Aromatase, 17 alpha-hydroxylase, and cholesterol side-chain cleavage P-450 cytochromes (P-450AROM, P-450(17 alpha,) and P-450SCC, respectively) were immunohistochemically localized in nine granulosa cell tumors, 15 thecomas, ten Sertoli-Leydig cell tumors, two steroid cell tumors, five fibromas, and five sclerosing stromal tumors. In the thecomas, P-450SCC and P-450(17 alpha) were positive in luteinized theca cells and in cells with vacuolated cytoplasm, while P-450AROM was not observed. In the steroid cell tumors, all the P-450 cytochromes were intensely stained. P-450SCC and P-450(17 alpha) were present in cells with vacuolated cytoplasm in two cases of sclerosing stromal tumor. P-450AROM was weakly demonstrated in one of the granulosa cell tumors. P-450(17 alpha,) P-450SCC, and P-450AROM were all faintly stained in the Sertoli-Leydig cell tumors. No P-450 cytochrome immunoreactivity was observed in any fibroma.

• Authors: H Sasano, M Okamoto, J I Mason, E R Simpson, C R Mendelson, N Sasano, S G Silverberg
• Journal: Human pathology (Hum Pathol) Vol. 20 Issue 5 Pg. 452-7 (May 1989) ISSN: 0046-8177 [Print] United States
• PMID: 2540088 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Steroid Hydroxylases
• Aromatase
• Steroid 17-alpha-Hydroxylase
• Cholesterol Side-Chain Cleavage Enzyme

Topics

• Aromatase (metabolism)
• Cholesterol Side-Chain Cleavage Enzyme (metabolism)
• Female
• Fibroma (enzymology)
• Granulosa Cell Tumor (enzymology)
• Humans
• Immunohistochemistry
• Leydig Cell Tumor (enzymology)
• Ovarian Neoplasms (enzymology)
• Sertoli Cell Tumor (enzymology)
• Steroid 17-alpha-Hydroxylase (metabolism)
• Steroid Hydroxylases (metabolism)
• Thecoma (enzymology)