In vivo proton MR spectroscopy of pancreatic neuroendocrine tumors in a multiple endocrine neoplasia type 1 conditional knockout mouse model.

Abstract

PURPOSE:

MR spectroscopy (MRS) can improve diagnosis and follow treatment in cancer. However, no study has yet reported application of in vivo (1)H-MRS in malignant pancreatic lesions. This study quantitatively determined whether in vivo (1)H-MRS on multiple endocrine neoplasia type 1 (Men1) conditional knockout (KO) mice and their wild type (WT) littermates could detect differences in total choline (tCho) levels between tumor and control pancreas.

METHODS:

Relative tCho levels in pancreatic tumors or pancreata from KO and WT mice were determined using in vivo (1)H-MRS at 9.4 T. The levels of Cho-containing compounds were also quantified using in vitro (1)H-NMR on extracts of pancreatic tissues from KO and WT mice, respectively, and on extracts of pancreatic tissues from patients with pancreatic neuroendocrine tumors (PNETs).

RESULTS:

tCho levels measured by in vivo (1)H-MRS were significantly higher in PNETs from KO mice compared to the normal pancreas from WT mice. The elevated choline-containing compounds were also identified in pancreatic tumors from KO mice and tissues from patients with PNETs via in vitro (1)H-NMR.

CONCLUSION:

These results indicate the potential use of tCho levels estimated via in vivo (1)H-MRS in differentiating malignant pancreatic tumors from benign tumors.

• Authors: Min-Hui Cui, Craig A Branch, Sean M Cahill, Thomas J Quinn, Asha Adem, Steven K Libutti, Ziqiang Yuan
• Journal: Magnetic resonance in medicine (Magn Reson Med) Vol. 74 Issue 5 Pg. 1221-6 (Nov 2015) ISSN: 1522-2594 [Electronic] United States
• PMID: 25392979 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Wiley Periodicals, Inc.

Chemical References

• Transcription Factors
• Choline

Topics

• Animals
• Choline (analysis, chemistry, metabolism)
• Female
• Magnetic Resonance Imaging (methods)
• Male
• Mice
• Mice, Knockout
• Multiple Endocrine Neoplasia Type 1 (chemistry, diagnosis, pathology)
• Pancreatic Neoplasms (chemistry, diagnosis, pathology)
• Transcription Factors (genetics)