The efficacy of antioxidant therapy against oxidative stress and androgen rise in ethylene glycol induced nephrolithiasis in Wistar rats.

Abstract

Administration of natural antioxidants has been used to protect against nephrolithiasis. Urolithiasis was induced by ethylene glycol (EG) in Wistar rats. For 4 weeks, group 1 (control) was fed with a standard commercial diet. Group 2 received the same diet with 0.75% of EG. Group 3 received EG plus the diet and water added with antioxidant nutrients and lime juice as the dietary source of citrate (EG + AX). Group 4 same as group 3 with no EG in water. For 8 weeks, group 5 was fed the standard diet with EG in water for the first 28 days, followed by no EG. Group 6 received the diet with EG for the first 28 days, followed by discontinuation of EG and addition of antioxidant nutrients. Group 7 were provided the diet with antioxidant nutrients for 8 weeks. Group 8 received the diet with antioxidant nutrients for 4 weeks, followed by antioxidant nutrients with EG for the next 4 weeks. Blood samples were collected and kidneys were removed. The size and the mean number of crystal deposits in EG-treated groups was significantly higher than the EG-treated groups, added with antioxidant nutrients and lime juice. After 4 weeks, the mean concentration of malondialdehyde in group 2 was higher than the group 3, and significantly lower in group 4; and in groups 7 after 8 weeks, as well. After 8 weeks, supplementation developed less mean number of deposits in group 6 as compared to group 5; and in group 8, the crystal deposits was substantially less than either group 2 or group 5 (EG-treated rats). Elevated concentration of androgens (as promoters of the formation of renal calculi) as a result of EG consumption decreased following antioxidant supplementations. Results showed a beneficial effect of antioxidant and provided superior renal protection on treating and preventing stone deposition in the rat kidney.

• Authors: M R Naghii, M Jafari, M Mofid, E Eskandari, M Hedayati, K Khalagie
• Journal: Human & experimental toxicology (Hum Exp Toxicol) Vol. 34 Issue 7 Pg. 744-54 (Jul 2015) ISSN: 1477-0903 [Electronic] England
• PMID: 25392345 (Publication Type: Journal Article)
• Copyright: © The Author(s) 2014.

Chemical References

• Antioxidants
• Sex Hormone-Binding Globulin
• Vitamins
• Dihydrotestosterone
• Vitamin A
• Vitamin E
• Testosterone
• Malondialdehyde
• Vitamin B 6
• Ethylene Glycol
• Selenium
• Zinc
• Boron
• Ascorbic Acid

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Ascorbic Acid (pharmacology, therapeutic use)
• Boron (pharmacology, therapeutic use)
• Dihydrotestosterone (blood)
• Ethylene Glycol
• Kidney (drug effects, pathology)
• Malondialdehyde (blood)
• Nephrolithiasis (blood, chemically induced, drug therapy, pathology)
• Oxidative Stress (drug effects)
• Rats, Wistar
• Selenium (pharmacology, therapeutic use)
• Sex Hormone-Binding Globulin (analysis)
• Testosterone (blood)
• Treatment Outcome
• Vitamin A (pharmacology, therapeutic use)
• Vitamin B 6 (pharmacology, therapeutic use)
• Vitamin E (pharmacology, therapeutic use)
• Vitamins (pharmacology, therapeutic use)
• Zinc (pharmacology, therapeutic use)