Abstract |
The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/ protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the concentrations of protein, TNF-α, and IL-6 in BALF, attenuated ALI, and increased PI3K and p-Akt expression in the lung tissue of LPS-challenged rats, whereas treatment with wortmannin, a PI3K/Akt pathway specific inhibitor, blunted this effect. Our study indicates that propofol pretreatment attenuated LPS-induced ALI, partly by activation of the PI3K/Akt pathway.
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Authors | L L Zhao, G C Hu, S S Zhu, J F Li, G J Liu |
Journal | Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
(Braz J Med Biol Res)
Vol. 47
Issue 12
Pg. 1062-7
(Dec 2014)
ISSN: 1414-431X [Electronic] Brazil |
PMID | 25387673
(Publication Type: Journal Article)
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Chemical References |
- Indicators and Reagents
- Interleukin-6
- Lipopolysaccharides
- Quinolines
- Tumor Necrosis Factor-alpha
- bicinchoninic acid
- Phosphatidylinositol 3-Kinases
- Proto-Oncogene Proteins c-akt
- Propofol
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Topics |
- Acute Lung Injury
(chemically induced, drug therapy, enzymology, metabolism)
- Animals
- Blotting, Western
- Bronchoalveolar Lavage Fluid
(chemistry)
- Enzyme-Linked Immunosorbent Assay
- Indicators and Reagents
- Interleukin-6
(analysis)
- Kaplan-Meier Estimate
- Lipopolysaccharides
- Lung
(drug effects, metabolism)
- Male
- Phosphatidylinositol 3-Kinases
(metabolism)
- Propofol
(metabolism, therapeutic use)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Quinolines
- Rats, Sprague-Dawley
- Signal Transduction
(drug effects)
- Tumor Necrosis Factor-alpha
(metabolism)
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