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Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway.

Abstract
The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the concentrations of protein, TNF-α, and IL-6 in BALF, attenuated ALI, and increased PI3K and p-Akt expression in the lung tissue of LPS-challenged rats, whereas treatment with wortmannin, a PI3K/Akt pathway specific inhibitor, blunted this effect. Our study indicates that propofol pretreatment attenuated LPS-induced ALI, partly by activation of the PI3K/Akt pathway.
AuthorsL L Zhao, G C Hu, S S Zhu, J F Li, G J Liu
JournalBrazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (Braz J Med Biol Res) Vol. 47 Issue 12 Pg. 1062-7 (Dec 2014) ISSN: 1414-431X [Electronic] Brazil
PMID25387673 (Publication Type: Journal Article)
Chemical References
  • Indicators and Reagents
  • Interleukin-6
  • Lipopolysaccharides
  • Quinolines
  • Tumor Necrosis Factor-alpha
  • bicinchoninic acid
  • Phosphatidylinositol 3-Kinases
  • Proto-Oncogene Proteins c-akt
  • Propofol
Topics
  • Acute Lung Injury (chemically induced, drug therapy, enzymology, metabolism)
  • Animals
  • Blotting, Western
  • Bronchoalveolar Lavage Fluid (chemistry)
  • Enzyme-Linked Immunosorbent Assay
  • Indicators and Reagents
  • Interleukin-6 (analysis)
  • Kaplan-Meier Estimate
  • Lipopolysaccharides
  • Lung (drug effects, metabolism)
  • Male
  • Phosphatidylinositol 3-Kinases (metabolism)
  • Propofol (metabolism, therapeutic use)
  • Proto-Oncogene Proteins c-akt (metabolism)
  • Quinolines
  • Rats, Sprague-Dawley
  • Signal Transduction (drug effects)
  • Tumor Necrosis Factor-alpha (metabolism)

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