Quantitation of putative glycoprotein X in bioengineered pseudorabies vaccine virus culture medium by ELISA.

Abstract

An enzyme-linked immunosorbent assay has been developed for the detection and quantitation of putative pseudorabies glycoprotein X (gX) in bulk bioengineered PRV delta gX delta tk-1 pseudorabies vaccine virus culture medium supernatants. The assay has a dynamic range of 0.2-25 ng, with a best linear region of 0.4-12.5 ng (correlation coefficient = 0.99) which permits 1 ppm discrimination for gX.

• Authors: L C Eaton
• Journal: Journal of immunological methods (J Immunol Methods) Vol. 118 Issue 1 Pg. 125-8 (Mar 10 1989) ISSN: 0022-1759 [Print] Netherlands
• PMID: 2538511 (Publication Type: Journal Article)

Chemical References

• Antigens, Viral
• Culture Media
• Vaccines
• Vaccines, Synthetic
• Viral Envelope Proteins
• Viral Vaccines
• pseudorabies virus glycoproteins

Topics

• Antigens, Viral (analysis)
• Culture Media (analysis)
• Enzyme-Linked Immunosorbent Assay (methods)
• Herpesvirus 1, Suid (immunology)
• Vaccines (analysis)
• Vaccines, Synthetic (analysis)
• Viral Envelope Proteins (analysis)
• Viral Vaccines (analysis)