Canthin-6-one displays antiproliferative activity and causes accumulation of cancer cells in the G2/M phase.
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| Abstract |
Canthinones are natural substances with a wide range of biological activities, including antipyretic, antiparasitic, and antimicrobial. Antiproliferative and/or cytotoxic effects of canthinones on cancer cells have also been described, although their mechanism of action remains ill defined. To gain better insight into this mechanism, the antiproliferative effect of a commercially available canthin-6-one (1) was examined dose-dependently on six cancer cell lines (human prostate, PC-3; human colon, HT-29; human lymphocyte, Jurkat; human cervix, HeLa; rat glioma, C6; and mouse embryonic fibroblasts, NIH-3T3). Cytotoxic effects of 1 were investigated on the same cancer cell lines by procaspase-3 cleavage and on normal human skin fibroblasts. Strong antiproliferative effects of the compound were observed in all cell lines, whereas cytotoxic effects were very dependent on cell type. A better definition of the mechanism of action of 1 was obtained on PC-3 cells, by showing that it decreases BrdU incorporation into DNA by 60% to 80% and mitotic spindle formation by 70% and that it causes a 2-fold accumulation of cells in the G2/M phase of the cell cycle. Together, the data suggest that the primary effect of canthin-6-one (1) is antiproliferative, possibly by interfering with the G2/M transition. Proapoptotic effects might result from this disturbance of the cell cycle.
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| Authors | Camille Dejos, Pierre Voisin, Marianne Bernard, Matthieu Régnacq, Thierry Bergès |
| Journal | Journal of natural products (J Nat Prod) Vol. 77 Issue 11 Pg. 2481-7 (Nov 26 2014) ISSN: 1520-6025 [Electronic] United States |
| PMID | 25379743 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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