Abstract | OBJECTIVES: MATERIALS AND METHODS:
Protein expression of pEGFR, VEGFR, NFκB, IKKα, IKKβ, MMP-2, MMP-9 and TIMP-2 were analysed by western blotting. We also analysed expressions of uPA, uPAR genes and chemokines by real-time PCR. Breast cancer cell invasion was assessed by transwell invasion assay and cell migration analysed by scratch wound healing assay. RESULTS: Our results showed that reduced protein expression of pEGFR, VEGFR, NFκB, IKKα, β, MMP-2, MMP-9 and TIMP-2 was higher in nimbolide-treated breast cancer cells. mRNA expression of uPA, uPAR, chemokines and their receptors were also significantly reduced in response to nimbolide treatment. Nimbolide inhibited breast cancer cell migration and invasion as shown in transwell invasion and wound healing assays. CONCLUSION: These results clearly proved inhibitory effects of nimbolide on tumour cell invasion and migration by down-regulating proteins critically involved in regulation of cell invasion and metastasis, suggesting a possible therapeutic role of nimbolide for breast cancer.
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Authors | P Elumalai, A Brindha Mercy, R Arunkamar, G Sharmila, F A Bhat, S Balakrishnan, P Raja Singh, J Arunakaran |
Journal | Cell proliferation
(Cell Prolif)
Vol. 47
Issue 6
Pg. 540-52
(Dec 2014)
ISSN: 1365-2184 [Electronic] England |
PMID | 25377085
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 John Wiley & Sons Ltd. |
Chemical References |
- Chemokines
- Limonins
- RNA, Messenger
- Receptors, Urokinase Plasminogen Activator
- Tissue Inhibitor of Metalloproteinase-2
- nimbolide
- Urokinase-Type Plasminogen Activator
- Matrix Metalloproteinase 2
- Matrix Metalloproteinase 9
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Topics |
- Breast Neoplasms
(metabolism, pathology)
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Chemokines
(genetics, metabolism)
- Down-Regulation
(drug effects)
- Female
- Humans
- Limonins
(toxicity)
- MCF-7 Cells
- Matrix Metalloproteinase 2
(genetics, metabolism)
- Matrix Metalloproteinase 9
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Receptors, Urokinase Plasminogen Activator
(genetics, metabolism)
- Tissue Inhibitor of Metalloproteinase-2
(genetics, metabolism)
- Urokinase-Type Plasminogen Activator
(genetics, metabolism)
|