Abstract | PURPOSE: MATERIALS AND METHODS: Using the streptavidin- peroxidase (SP) immunohistochemical method, paraffin sections from 100 colorectal cancer samples and 10 samples from tumor-adjacent normal tissue (>2 cm from the edge of the gross tumor) were tested for protein expression of Ang-2, Tie-2, PI3K, and AKT. Reverse transcription-polymerase chain reaction and Western blots were further used to measure expression of the 4 genes and proteins in 20 freshly-resected colorectal cancer samples and tumor-adjacent normal tissues. RESULTS: CONCLUSIONS: In colorectal cancer, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins is markedly higher than those in tumor-adjacent normal tissues. No correlation was observed between protein expression and gender, location, or histologic type. Correlations did exist between protein expression and differentiation level, stage of Duke's classification, and lymphatic metastasis; in colorectal cancer tissues with lower differentiation levels, higher stages of Duke's classification, and lymphatic metastasis, the expression of all 4 proteins was higher. The study of their expression patterns and relationships with aggression and metastasis will provide a valuable experimental foundation for assessing prognosis and targeted therapy of colorectal cancer.
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Authors | Ji-Hong Zhang, Li-Hua Wang, Xiang-Jun Li, Ai-Ping Wang, Li-Qun Reng, Feng-Guo Xia, Zhi-Ping Yang, Jing Jiang, Xiao-Dan Wang, Chun-Yang Wen |
Journal | Asian Pacific journal of cancer prevention : APJCP
(Asian Pac J Cancer Prev)
Vol. 15
Issue 20
Pg. 8651-6
( 2014)
ISSN: 2476-762X [Electronic] Thailand |
PMID | 25374184
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- RNA, Messenger
- Phosphatidylinositol 3-Kinases
- Receptor, TIE-2
- Oncogene Protein v-akt
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Topics |
- Adenocarcinoma
(genetics, pathology, surgery)
- Adult
- Aged
- Blotting, Western
- Case-Control Studies
- Colorectal Neoplasms
(genetics, pathology, surgery)
- Disease Progression
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Oncogene Protein v-akt
(genetics)
- Phosphatidylinositol 3-Kinases
(genetics)
- Predictive Value of Tests
- RNA, Messenger
(analysis)
- Real-Time Polymerase Chain Reaction
(methods)
- Receptor, TIE-2
(genetics)
- Tissue Embedding
- Up-Regulation
(genetics)
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