HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Expression of Ang-2/Tie-2 and PI3K/AKT in colorectal cancer.

AbstractPURPOSE:
To study the expression of angiogenin-2 (Ang-2) and its receptor Tie-2 in colorectal cancer and discuss the possible mechanisms behind this process.
MATERIALS AND METHODS:
Using the streptavidin-peroxidase (SP) immunohistochemical method, paraffin sections from 100 colorectal cancer samples and 10 samples from tumor-adjacent normal tissue (>2 cm from the edge of the gross tumor) were tested for protein expression of Ang-2, Tie-2, PI3K, and AKT. Reverse transcription-polymerase chain reaction and Western blots were further used to measure expression of the 4 genes and proteins in 20 freshly-resected colorectal cancer samples and tumor-adjacent normal tissues.
RESULTS:
In colorectal cancer tissues, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins was significantly higher than in tumor-adjacent normal tissues. Protein expression in poorly-differentiated adenocarcinoma was higher than that in well and moderately differentiated adenocarcinoma. According to Duke's classification, the protein expression in Stages C and D was significantly higher than that in Stages A and B. In the group with lymphatic metastasis, the protein expression was higher than that without lymphatic metastasis.
CONCLUSIONS:
In colorectal cancer, the expression of the Ang-2, Tie-2, PI3K, and AKT genes and their proteins is markedly higher than those in tumor-adjacent normal tissues. No correlation was observed between protein expression and gender, location, or histologic type. Correlations did exist between protein expression and differentiation level, stage of Duke's classification, and lymphatic metastasis; in colorectal cancer tissues with lower differentiation levels, higher stages of Duke's classification, and lymphatic metastasis, the expression of all 4 proteins was higher. The study of their expression patterns and relationships with aggression and metastasis will provide a valuable experimental foundation for assessing prognosis and targeted therapy of colorectal cancer.
AuthorsJi-Hong Zhang, Li-Hua Wang, Xiang-Jun Li, Ai-Ping Wang, Li-Qun Reng, Feng-Guo Xia, Zhi-Ping Yang, Jing Jiang, Xiao-Dan Wang, Chun-Yang Wen
JournalAsian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 15 Issue 20 Pg. 8651-6 ( 2014) ISSN: 1513-7368 [Print] Thailand
PMID25374184 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • RNA, Messenger
  • Phosphatidylinositol 3-Kinases
  • Receptor, TIE-2
  • Oncogene Protein v-akt
Topics
  • Adenocarcinoma (genetics, pathology, surgery)
  • Adult
  • Aged
  • Blotting, Western
  • Case-Control Studies
  • Colorectal Neoplasms (genetics, pathology, surgery)
  • Disease Progression
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Oncogene Protein v-akt (genetics)
  • Phosphatidylinositol 3-Kinases (genetics)
  • Predictive Value of Tests
  • RNA, Messenger (analysis)
  • Real-Time Polymerase Chain Reaction (methods)
  • Receptor, TIE-2 (genetics)
  • Tissue Embedding
  • Up-Regulation (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: