Bioactivity-guided isolation of anticancer agents from Bauhinia kockiana Korth.

Abstract

BACKGROUND:

Flowers of Bauhinia kockiana were investigated for their anticancer properties.

METHODS:

Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds.

RESULTS:

The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50 30 - 86 µM), but were inactive towards DU145 prostate cancer cell (GI50 > 100 µM).

CONCLUSION:

It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound 1 was also important in anticancer activity. Examination of the PC-3 cells treated with compound 1 using fluorescence microscopy showed that PC-3 cells were killed by apoptosis.

• Authors: Yik Ling Chew, Yau Yan Lim, Johnson Stanslas, Gwendoline Cheng Lian Ee, Joo Kheng Goh
• Journal: African journal of traditional, complementary, and alternative medicines : AJTCAM (Afr J Tradit Complement Altern Med) Vol. 11 Issue 3 Pg. 291-9 ( 2014) ISSN: 2505-0044 [Electronic] Nigeria
• PMID: 25371595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Phenols
• Plant Extracts

Topics

• Antineoplastic Agents (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Bauhinia (chemistry)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Humans
• Male
• Phenols (isolation & purification, pharmacology)
• Plant Extracts (isolation & purification, pharmacology)
• Prostatic Neoplasms (drug therapy, physiopathology)