Local evolution of pyrethroid resistance offsets gene flow among Aedes aegypti collections in Yucatan State, Mexico.

Abstract

The mosquito Aedes aegypti is the major vector of the four serotypes of dengue virus (DENV1-4). Previous studies have shown that Ae. aegypti in Mexico have a high effective migration rate and that gene flow occurs among populations that are up to 150 km apart. Since 2000, pyrethroids have been widely used for suppression of Ae. aegypti in cities in Mexico. In Yucatan State in particular, pyrethroids have been applied in and around dengue case households creating an opportunity for local selection and evolution of resistance. Herein, we test for evidence of local adaptation by comparing patterns of variation among 27 Ae. aegypti collections at 13 single nucleotide polymorphisms (SNPs): two in the voltage-gated sodium channel gene para known to confer knockdown resistance, three in detoxification genes previously associated with pyrethroid resistance, and eight in putatively neutral loci. The SNPs in para varied greatly in frequency among collections, whereas SNPs at the remaining 11 loci showed little variation supporting previous evidence for extensive local gene flow. Among Ae. aegypti in Yucatan State, Mexico, local adaptation to pyrethroids appears to offset the homogenizing effects of gene flow.

• Authors: Karla Saavedra-Rodriguez, Meaghan Beaty, Saul Lozano-Fuentes, Steven Denham, Julian Garcia-Rejon, Guadalupe Reyes-Solis, Carlos Machain-Williams, Maria Alba Loroño-Pino, Adriana Flores-Suarez, Gustavo Ponce-Garcia, Barry Beaty, Lars Eisen, William C Black 4th
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 92 Issue 1 Pg. 201-9 (Jan 2015) ISSN: 1476-1645 [Electronic] United States
• PMID: 25371186 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © The American Society of Tropical Medicine and Hygiene.

Chemical References

• Pyrethrins

Topics

• Aedes (parasitology)
• Animals
• Insect Vectors
• Insecticide Resistance (genetics)
• Mexico
• Pyrethrins (pharmacology)