Quantitative peptidomics study reveals that a wound-induced peptide from PR-1 regulates immune signaling in tomato.

Abstract

Many important cell-to-cell communication events in multicellular organisms are mediated by peptides, but only a few peptides have been identified in plants. In an attempt to address the difficulties in identifying plant signaling peptides, we developed a novel peptidomics approach and used this approach to discover defense signaling peptides in plants. In addition to the canonical peptide systemin, several novel peptides were confidently identified in tomato (Solanum lycopersicum) and quantified to be induced by both wounding and methyl jasmonate (MeJA). A wounding or wounding plus MeJA-induced peptide derived from the pathogenesis-related protein 1 (PR-1) family was found to induce significant antipathogen and minor antiherbivore responses in tomato. This study highlights a role for PR-1 in immune signaling and suggests the potential application of plant endogenous peptides in efforts to defeat biological threats in crop production. As PR-1 is highly conserved across many organisms and the putative peptide from At-PR1 was also found to be bioactive in Arabidopsis thaliana, our results suggest that this peptide may be useful for enhancing resistance to stress in other plant species.

• Authors: Ying-Lan Chen, Chi-Ying Lee, Kai-Tan Cheng, Wei-Hung Chang, Rong-Nan Huang, Hong Gil Nam, Yet-Ran Chen
• Journal: The Plant cell (Plant Cell) Vol. 26 Issue 10 Pg. 4135-48 (Oct 2014) ISSN: 1532-298X [Electronic] England
• PMID: 25361956 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 American Society of Plant Biologists. All rights reserved.

Chemical References

• Acetates
• Cyclopentanes
• Oxylipins
• Peptides
• Plant Growth Regulators
• Plant Proteins
• Proteome
• pathogenesis-related proteins, plant
• methyl jasmonate

Topics

• Acetates (pharmacology)
• Amino Acid Sequence
• Chromatography, Liquid
• Cyclopentanes (pharmacology)
• Disease Resistance (drug effects, genetics, immunology)
• Host-Pathogen Interactions (drug effects, immunology)
• Solanum lycopersicum (genetics, metabolism, microbiology)
• Molecular Sequence Data
• Oligonucleotide Array Sequence Analysis
• Oxylipins (pharmacology)
• Peptides (genetics, metabolism, pharmacology)
• Plant Diseases (genetics, microbiology)
• Plant Growth Regulators (pharmacology)
• Plant Leaves (genetics, metabolism, microbiology)
• Plant Proteins (chemistry, genetics, metabolism)
• Proteome (genetics, metabolism, pharmacology)
• Proteomics
• Pseudomonas syringae (immunology, physiology)
• Reverse Transcriptase Polymerase Chain Reaction
• Stress, Mechanical
• Tandem Mass Spectrometry
• Transcriptome (drug effects, genetics, immunology)