Abstract |
A total of 85 Uropathogenic Escherichia coli (UPEC) isolates were screened against ceftiofur, oxacillin, nitrofurantoin and lincospectin using Kirby-Bauer disc diffusion method, following CLSI guidelines. Prevalence of virulent factor genes amongst the isolates was determined by PCR, using gene-specific primers against the different virulent factors. Statistical analysis of the data was performed using SPSS software. The prevalence of traT, ompT, Iss, malX and ibeA genes was 47.1%, 38.8%, 20%, 16.5% and 9.4%, respectively. The most prevalent gene in group A and D was traT, whilst in group B2 was Iss. The highest resistance has been shown against oxacillin (98.8%), followed by ceftiofur (77.6%), whilst resistance to lincospectin (2.4%) and nitrofurantoin (12.9%) had the lowest frequencies. Multidrug resistance was shown in 82.35% of the isolates, whilst this study recommend lincospectin and nitrofurantoin as choice drugs for treatment, but more investigation of the bacterial pathogenicity associated with urinary tract infection (UTI) may contribute to a better medical intervention.
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Authors | A Derakhshandeh, R Firouzi, M Motamedifar, A Motamedi Boroojeni, M Bahadori, S Arabshahi, A Novinrooz, S Heidari |
Journal | Letters in applied microbiology
(Lett Appl Microbiol)
Vol. 60
Issue 2
Pg. 148-154
(Feb 2015)
ISSN: 1472-765X [Electronic] England |
PMID | 25355175
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 The Society for Applied Microbiology. |
Chemical References |
- Anti-Bacterial Agents
- Virulence Factors
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Disk Diffusion Antimicrobial Tests
- Drug Resistance, Multiple, Bacterial
- Escherichia coli Infections
(microbiology)
- Female
- Genes, Bacterial
- Humans
- Male
- Phylogeny
- Polymerase Chain Reaction
- Urinary Tract Infections
(microbiology)
- Uropathogenic Escherichia coli
(drug effects, genetics, isolation & purification, pathogenicity)
- Virulence
(genetics)
- Virulence Factors
(genetics)
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