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Distribution of virulence genes and multiple drug-resistant patterns amongst different phylogenetic groups of uropathogenic Escherichia coli isolated from patients with urinary tract infection.

Abstract
A total of 85 Uropathogenic Escherichia coli (UPEC) isolates were screened against ceftiofur, oxacillin, nitrofurantoin and lincospectin using Kirby-Bauer disc diffusion method, following CLSI guidelines. Prevalence of virulent factor genes amongst the isolates was determined by PCR, using gene-specific primers against the different virulent factors. Statistical analysis of the data was performed using SPSS software. The prevalence of traT, ompT, Iss, malX and ibeA genes was 47.1%, 38.8%, 20%, 16.5% and 9.4%, respectively. The most prevalent gene in group A and D was traT, whilst in group B2 was Iss. The highest resistance has been shown against oxacillin (98.8%), followed by ceftiofur (77.6%), whilst resistance to lincospectin (2.4%) and nitrofurantoin (12.9%) had the lowest frequencies. Multidrug resistance was shown in 82.35% of the isolates, whilst this study recommend lincospectin and nitrofurantoin as choice drugs for treatment, but more investigation of the bacterial pathogenicity associated with urinary tract infection (UTI) may contribute to a better medical intervention.
AuthorsA Derakhshandeh, R Firouzi, M Motamedifar, A Motamedi Boroojeni, M Bahadori, S Arabshahi, A Novinrooz, S Heidari
JournalLetters in applied microbiology (Lett Appl Microbiol) Vol. 60 Issue 2 Pg. 148-154 (Feb 2015) ISSN: 1472-765X [Electronic] England
PMID25355175 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2014 The Society for Applied Microbiology.
Chemical References
  • Anti-Bacterial Agents
  • Virulence Factors
Topics
  • Anti-Bacterial Agents (pharmacology)
  • Disk Diffusion Antimicrobial Tests
  • Drug Resistance, Multiple, Bacterial
  • Escherichia coli Infections (microbiology)
  • Female
  • Genes, Bacterial
  • Humans
  • Male
  • Phylogeny
  • Polymerase Chain Reaction
  • Urinary Tract Infections (microbiology)
  • Uropathogenic Escherichia coli (drug effects, genetics, isolation & purification, pathogenicity)
  • Virulence (genetics)
  • Virulence Factors (genetics)

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