Abstract | CONTEXT AND OBJECTIVE: DESIGN AND SETTING: This was an analytical cross-sectional study. Peripheral blood samples were collected at a Brazilian public hospital, from 21 patients with histopathological diagnoses of gastrointestinal carcinomas and from 43 healthy individuals. The analyses were performed in two Brazilian medical schools. METHODS:
Heparanase isoforms were identified and quantified in plasma samples by means of Western blot. The enzymatic activities of heparanase-1 and cathepsin B were also measured. RESULTS: The results demonstrated that the expression of both heparanase isoforms was significantly greater in plasma samples from gastrointestinal carcinoma patients, compared with the control group. Logistic regression analysis showed that increased heparanase-1 and heparanase-2 expression was exclusively dependent on the tumor. There was a significant increase in heparanase-1 and cathepsin B activity in the patients' plasma. CONCLUSION: Overexpression of heparanase-1 and heparanase-2, along with increased heparanase-1 and cathepsin B activity in plasma, is associated with the diagnosis of gastrointestinal carcinoma. These findings provide support for using non-invasive assays (plasma samples) as an auxiliary method for diagnosing gastrointestinal tumors.
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Authors | Carina Mucciolo Melo, Clarice Silvia Taemi Origassa, Thérèse Rachell Theodoro, Leandro Luongo Matos, Thaís Aguilar Miranda, Camila Melo Accardo, Rodrigo Ippolito Bouças, Eloah Rabello Suarez, Madalena Maria Nunes Silva Pares, Daniel Reis Waisberg, Giovanna Canato Toloi, Helena Bonciani Nader, Jaques Waisberg, Maria Aparecida Silva Pinhal |
Journal | Sao Paulo medical journal = Revista paulista de medicina
(Sao Paulo Med J)
Vol. 133
Issue 1
Pg. 28-35
(Feb 2015)
ISSN: 1806-9460 [Electronic] Brazil |
PMID | 25351637
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Isoenzymes
- heparanase
- Glucuronidase
- Cathepsin B
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Topics |
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(blood)
- Blotting, Western
(methods)
- Carcinoma
(enzymology)
- Case-Control Studies
- Cathepsin B
(blood)
- Cross-Sectional Studies
- Female
- Gastrointestinal Neoplasms
(enzymology)
- Glucuronidase
(blood)
- Humans
- Immunoenzyme Techniques
- Isoenzymes
(blood)
- Male
- Middle Aged
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