miR-382 inhibits tumor growth and enhance chemosensitivity in osteosarcoma.

Abstract

Dysregulation of miRNAs is involved in osteosarcoma (OS). Here, we demonstrate that miR-382 is decreased in specimens of OS patients with a poor chemoresponse compared to those with a good chemoresponse. In addition, our clinical data show that decreased miR-382 was associated with poor survival in OS patients. Overexpression of miR-382 inhibited cell growth and chemoresistance by targeting KLF12 and HIPK3, respectively. In contrast, inhibition of miR-382 or overexpression of target genes stimulated OS cell growth and chemoresistance both in vitro and in vivo. Taken together, these findings suggest that miR-382 is a tumor suppressor miRNA and induction of miR-382 is a potential strategy to inhibit OS progression.

• Authors: Meng Xu, Hua Jin, Cheng-Xiong Xu, Bo Sun, Zhi Mao, Wen-Zhi Bi, Yan Wang
• Journal: Oncotarget (Oncotarget) Vol. 5 Issue 19 Pg. 9472-83 (Oct 15 2014) ISSN: 1949-2553 [Electronic] United States
• PMID: 25344865 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intracellular Signaling Peptides and Proteins
• KLF12 protein, human
• Kruppel-Like Transcription Factors
• MIRN382 microRNA, human
• MicroRNAs
• HIPK3 protein, human
• Protein Serine-Threonine Kinases

Topics

• Animals
• Apoptosis (genetics)
• Bone Neoplasms (genetics, mortality, pathology)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Drug Resistance, Neoplasm (genetics)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Intracellular Signaling Peptides and Proteins (biosynthesis, genetics)
• Kruppel-Like Transcription Factors (biosynthesis, genetics)
• Mice
• Mice, Nude
• MicroRNAs (antagonists & inhibitors, biosynthesis, genetics)
• Osteosarcoma (drug therapy, genetics, mortality, pathology)
• Protein Serine-Threonine Kinases (biosynthesis, genetics)
• Treatment Outcome