Potential mechanisms of benzyl isothiocyanate suppression of invasion and angiogenesis by the U87MG human glioma cell line.

Abstract

Glioma is one of the most common tumors in China and chemotherapy is critical for its treatment. Recent studies showed that benzyl isothiocyanate (BITC) could inhibit the growth of glioma cells, but the mechanisms are not fully understood. This study explored the inhibitory effect of BITC on invasion and angiogenesis of U87MG human glioma cells in vitro and in vivo, as well as potential mechanisms. It was found that BITC could inhibit invasion and angiogenesis of human glioma U87MG cells by inducing cell cycle arrest at phase G2/M. It also was demonstrated that BITC decreased expression of cyclin B1, p21, MMP-2/9, VE-cadherin, CD44, CXCR4 and MTH1, the activity of the telomerase and PKCζ pathway. Microarray analysis was thus useful to explore the potential target genes related to tumorigenic processes. BITC may play important roles in the inhibition of invasion and angiogenesis of human glioma cells.

• Authors: Yu Zhu, Ling Zhang, Guo-Dong Zhang, Hong-Ou Wang, Ming-Yan Liu, Yuan Jiang, Li-Sha Qi, Qi Li, Ping Yang
• Journal: Asian Pacific journal of cancer prevention : APJCP (Asian Pac J Cancer Prev) Vol. 15 Issue 19 Pg. 8225-8 ( 2014) ISSN: 2476-762X [Electronic] Thailand
• PMID: 25339010 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Isothiocyanates
• RNA, Messenger
• benzyl isothiocyanate

Topics

• Apoptosis
• Biomarkers, Tumor (metabolism)
• Blotting, Western
• Brain Neoplasms (drug therapy, metabolism, pathology)
• Cell Adhesion
• Cell Cycle
• Cell Movement
• Cell Proliferation
• Gene Expression Profiling
• Glioma (drug therapy, metabolism, pathology)
• Humans
• Isothiocyanates (pharmacology)
• Neovascularization, Pathologic (prevention & control)
• RNA, Messenger (genetics)
• Real-Time Polymerase Chain Reaction
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction
• Tumor Cells, Cultured