Cocoon-like self-degradable DNA nanoclew for anticancer drug delivery.

Abstract

A bioinspired cocoon-like anticancer drug delivery system consisting of a deoxyribonuclease (DNase)-degradable DNA nanoclew (NCl) embedded with an acid-responsive DNase I nanocapsule (NCa) was developed for targeted cancer treatment. The NCl was assembled from a long-chain single-stranded DNA synthesized by rolling-circle amplification (RCA). Multiple GC-pair sequences were integrated into the NCl for enhanced loading capacity of the anticancer drug doxorubicin (DOX). Meanwhile, negatively charged DNase I was encapsulated in a positively charged acid-degradable polymeric nanogel to facilitate decoration of DNase I into the NCl by electrostatic interactions. In an acidic environment, the activity of DNase I was activated through the acid-triggered shedding of the polymeric shell of the NCa, resulting in the cocoon-like self-degradation of the NCl and promoting the release of DOX for enhanced therapeutic efficacy.

• Authors: Wujin Sun, Tianyue Jiang, Yue Lu, Margaret Reiff, Ran Mo, Zhen Gu
• Journal: Journal of the American Chemical Society (J Am Chem Soc) Vol. 136 Issue 42 Pg. 14722-5 (Oct 22 2014) ISSN: 1520-5126 [Electronic] United States
• PMID: 25336272 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Drug Carriers
• Doxorubicin
• DNA
• Deoxyribonucleases

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• DNA (chemistry, metabolism)
• Deoxyribonucleases (metabolism)
• Doxorubicin (chemistry, pharmacology)
• Drug Carriers (chemistry, metabolism)
• Drug Liberation
• Humans
• MCF-7 Cells
• Models, Molecular
• Molecular Conformation
• Nanoparticles (chemistry)